BRENDA - Enzyme Database
show all sequences of 3.1.2.29

Entropy drives selective fluorine recognition in the fluoroacetyl-CoA thioesterase from Streptomyces cattleya

Weeks, A.M.; Wang, N.; Pelton, J.G.; Chang, M.C.Y.; Proc. Natl. Acad. Sci. USA 115, E2193-E2201 (2018)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
F36A
the mutation results in a change in KM by affecting substrate binding
Streptomyces cattleya
Inhibitors
Inhibitors
Commentary
Organism
Structure
acetyl-aza(dethia)-CoA
-
Streptomyces cattleya
acetyl-carba(dethia)-CoA
-
Streptomyces cattleya
acetyl-oxa(dethia)-CoA
-
Streptomyces cattleya
fluoroacetyl-aza(dethia)-CoA
-
Streptomyces cattleya
fluoroacetyl-carba(dethia)-CoA
-
Streptomyces cattleya
fluoroacetyl-oxa(dethia)-CoA
-
Streptomyces cattleya
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
fluoroacetyl-CoA + H2O
Streptomyces cattleya
-
fluoroacetate + CoA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces cattleya
Q1EMV2
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + H2O
-
752061
Streptomyces cattleya
acetate + CoA
-
-
-
?
fluoroacetyl-CoA + H2O
-
752061
Streptomyces cattleya
fluoroacetate + CoA
-
-
-
?
fluoroacetyl-CoA + H2O
the enzyme is strongly selective for fluoroacetyl-CoA compared with acetyl-CoA
752061
Streptomyces cattleya
fluoroacetate + CoA
-
-
-
?
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.13
-
fluoroacetyl-oxa(dethia)-CoA
wild type enzyme, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
0.26
-
fluoroacetyl-oxa(dethia)-CoA
mutant enzyme F36A, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
0.3
-
acetyl-oxa(dethia)-CoA
mutant enzyme F36A, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
0.32
-
fluoroacetyl-oxa(dethia)-CoA
wild type enzyme, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
0.33
-
fluoroacetyl-oxa(dethia)-CoA
mutant enzyme F36A, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
0.46
-
fluoroacetyl-carba(dethia)-CoA
wild type enzyme, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
0.51
-
acetyl-oxa(dethia)-CoA
wild type enzyme, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
0.9
-
fluoroacetyl-carba(dethia)-CoA
wild type enzyme, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
1.2
-
fluoroacetyl-aza(dethia)-CoA
wild type enzyme, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
1.5
-
acetyl-oxa(dethia)-CoA
mutant enzyme F36A, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
2.1
-
fluoroacetyl-carba(dethia)-CoA
mutant enzyme F36A, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
2.5
-
acetyl-oxa(dethia)-CoA
wild type enzyme, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
2.6
-
acetyl-carba(dethia)-CoA
wild type enzyme, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
2.8
-
acetyl-aza(dethia)-CoA
mutant enzyme F36A, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
3.6
-
acetyl-carba(dethia)-CoA
mutant enzyme F36A, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
4
-
fluoroacetyl-aza(dethia)-CoA
mutant enzyme F36A, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
4.3
-
fluoroacetyl-aza(dethia)-CoA
wild type enzyme, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
4.5
-
fluoroacetyl-carba(dethia)-CoA
mutant enzyme F36A, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
4.7
-
acetyl-aza(dethia)-CoA
mutant enzyme F36A, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
7.5
-
fluoroacetyl-aza(dethia)-CoA
mutant enzyme F36A, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
8.4
-
acetyl-aza(dethia)-CoA
wild type enzyme, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
11
-
acetyl-carba(dethia)-CoA
wild type enzyme, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
33
-
acetyl-aza(dethia)-CoA
wild type enzyme, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
51
-
acetyl-carba(dethia)-CoA
mutant enzyme F36A, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
F36A
the mutation results in a change in KM by affecting substrate binding
Streptomyces cattleya
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetyl-aza(dethia)-CoA
-
Streptomyces cattleya
acetyl-carba(dethia)-CoA
-
Streptomyces cattleya
acetyl-oxa(dethia)-CoA
-
Streptomyces cattleya
fluoroacetyl-aza(dethia)-CoA
-
Streptomyces cattleya
fluoroacetyl-carba(dethia)-CoA
-
Streptomyces cattleya
fluoroacetyl-oxa(dethia)-CoA
-
Streptomyces cattleya
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.13
-
fluoroacetyl-oxa(dethia)-CoA
wild type enzyme, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
0.26
-
fluoroacetyl-oxa(dethia)-CoA
mutant enzyme F36A, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
0.3
-
acetyl-oxa(dethia)-CoA
mutant enzyme F36A, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
0.32
-
fluoroacetyl-oxa(dethia)-CoA
wild type enzyme, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
0.33
-
fluoroacetyl-oxa(dethia)-CoA
mutant enzyme F36A, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
0.46
-
fluoroacetyl-carba(dethia)-CoA
wild type enzyme, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
0.51
-
acetyl-oxa(dethia)-CoA
wild type enzyme, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
0.9
-
fluoroacetyl-carba(dethia)-CoA
wild type enzyme, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
1.2
-
fluoroacetyl-aza(dethia)-CoA
wild type enzyme, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
1.5
-
acetyl-oxa(dethia)-CoA
mutant enzyme F36A, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
2.1
-
fluoroacetyl-carba(dethia)-CoA
mutant enzyme F36A, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
2.5
-
acetyl-oxa(dethia)-CoA
wild type enzyme, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
2.6
-
acetyl-carba(dethia)-CoA
wild type enzyme, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
2.8
-
acetyl-aza(dethia)-CoA
mutant enzyme F36A, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
3.6
-
acetyl-carba(dethia)-CoA
mutant enzyme F36A, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
4
-
fluoroacetyl-aza(dethia)-CoA
mutant enzyme F36A, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
4.3
-
fluoroacetyl-aza(dethia)-CoA
wild type enzyme, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
4.5
-
fluoroacetyl-carba(dethia)-CoA
mutant enzyme F36A, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
4.7
-
acetyl-aza(dethia)-CoA
mutant enzyme F36A, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
7.5
-
fluoroacetyl-aza(dethia)-CoA
mutant enzyme F36A, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
8.4
-
acetyl-aza(dethia)-CoA
wild type enzyme, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
11
-
acetyl-carba(dethia)-CoA
wild type enzyme, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
33
-
acetyl-aza(dethia)-CoA
wild type enzyme, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
51
-
acetyl-carba(dethia)-CoA
mutant enzyme F36A, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication
Streptomyces cattleya
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
fluoroacetyl-CoA + H2O
Streptomyces cattleya
-
fluoroacetate + CoA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + H2O
-
752061
Streptomyces cattleya
acetate + CoA
-
-
-
?
fluoroacetyl-CoA + H2O
-
752061
Streptomyces cattleya
fluoroacetate + CoA
-
-
-
?
fluoroacetyl-CoA + H2O
the enzyme is strongly selective for fluoroacetyl-CoA compared with acetyl-CoA
752061
Streptomyces cattleya
fluoroacetate + CoA
-
-
-
?
Other publictions for EC 3.1.2.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752061
Weeks
Entropy drives selective fluo ...
Streptomyces cattleya
Proc. Natl. Acad. Sci. USA
115
E2193-E2201
2018
-
-
-
-
1
-
6
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
24
-
-
-
-
-
-
-
1
-
-
6
24
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729250
Weeks
Molecular recognition of fluor ...
Streptomyces cattleya
Biochemistry
53
2053-2063
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730813
Weeks
Catalytic control of enzymatic ...
Streptomyces cattleya
Proc. Natl. Acad. Sci. USA
109
19667-19672
2012
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
711265
Weeks
Structural and biochemical stu ...
Streptomyces cattleya
Biochemistry
49
9269-9279
2010
-
-
-
1
9
-
-
15
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
-
1
9
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
13
-
-
-
-
-
1
1
-
15
15
712448
Dias
Structural basis for the activ ...
Streptomyces cattleya
J. Biol. Chem.
285
22495-22504
2010
-
-
1
1
4
-
1
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
1
4
-
-
1
-
3
-
-
-
-
-
-
-
1
-
-
-
-
2
1
-
-
-
3
-
-
-
-
-
-
-
-
3
3
712861
Meyer
-
Fluoroacetyl-coenzyme A hydrol ...
Dichapetalum cymosum
J. Plant Physiol.
139
369-372
1992
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-