Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, and in developing oilseeds of Arabidopsis thaliana and Brassica napus | Umbellularia californica |
Protein Variants | Comment | Organism |
---|---|---|
M197E/E199H | the double mutant M197R/R199H has equal preference for dodecanoyl-[acyl-carrier protein] and tetradecanoyl-[acyl-carrier protein]. Mutant T231K shows no altered specificity. Mutant R327Q and R322M/R327Q have specific activities towards dodecanoyl-[acyl-carrier protein] and tetradecanoyl-[acyl-carrier protein] decreased about 100fold and 30fold. The triple mutant M197R/R199H/T231K is a tetradecanoyl-[acyl-carrier protein] thioesterase | Umbellularia californica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dodecanoyl-[acyl-carrier protein] | competitive with respect to tetradecanoyl-[acyl-carrier protein], wild type enzyme and mutant enzyme M197R/R199H/T123K | Umbellularia californica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
dodecanoyl-[acyl-carrier protein] | wild type enzyme | Umbellularia californica | |
0.0023 | - |
tetradecanoyl-[acyl-carrier protein] | triple mutant M197R/R199H/T231K | Umbellularia californica | |
0.0064 | - |
tetradecanoyl-[acyl-carrier protein] | wild type enzyme | Umbellularia californica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Umbellularia californica | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dodecanoyl-[acyl-carrier protein] + H2O | the C-terminal two-third of the protein is critical for the specificity | Umbellularia californica | [acyl-carrier protein] + dodecanoate | - |
? | |
tetradecanoyl-[acyl-carrier protein] + H2O | the C-terminal two-third of the protein is critical for the specificity | Umbellularia californica | [acyl-carrier protein] + tetradecanoate | - |
? |