BRENDA - Enzyme Database show
show all sequences of 3.1.2.21

Modification of the substrate specificity of an acyl-acyl carrier protein thioesterase by protein engineering

Yuan, L.; Voelker, T.A.; Hawkins, D.J.; Proc. Natl. Acad. Sci. USA 92, 10639-10643 (1995)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli, and in developing oilseeds of Arabidopsis thaliana and Brassica napus
Umbellularia californica
Engineering
Amino acid exchange
Commentary
Organism
M197E/E199H
the double mutant M197R/R199H has equal preference for dodecanoyl-[acyl-carrier protein] and tetradecanoyl-[acyl-carrier protein]. Mutant T231K shows no altered specificity. Mutant R327Q and R322M/R327Q have specific activities towards dodecanoyl-[acyl-carrier protein] and tetradecanoyl-[acyl-carrier protein] decreased about 100fold and 30fold. The triple mutant M197R/R199H/T231K is a tetradecanoyl-[acyl-carrier protein] thioesterase
Umbellularia californica
Inhibitors
Inhibitors
Commentary
Organism
Structure
dodecanoyl-[acyl-carrier protein]
competitive with respect to tetradecanoyl-[acyl-carrier protein], wild type enzyme and mutant enzyme M197R/R199H/T123K
Umbellularia californica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0019
-
dodecanoyl-[acyl-carrier protein]
wild type enzyme
Umbellularia californica
0.0023
-
tetradecanoyl-[acyl-carrier protein]
triple mutant M197R/R199H/T231K
Umbellularia californica
0.0064
-
tetradecanoyl-[acyl-carrier protein]
wild type enzyme
Umbellularia californica
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Umbellularia californica
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dodecanoyl-[acyl-carrier protein] + H2O
the C-terminal two-third of the protein is critical for the specificity
23907
Umbellularia californica
[acyl-carrier protein] + dodecanoate
-
-
-
-
tetradecanoyl-[acyl-carrier protein] + H2O
the C-terminal two-third of the protein is critical for the specificity
23907
Umbellularia californica
[acyl-carrier protein] + tetradecanoate
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli, and in developing oilseeds of Arabidopsis thaliana and Brassica napus
Umbellularia californica
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
M197E/E199H
the double mutant M197R/R199H has equal preference for dodecanoyl-[acyl-carrier protein] and tetradecanoyl-[acyl-carrier protein]. Mutant T231K shows no altered specificity. Mutant R327Q and R322M/R327Q have specific activities towards dodecanoyl-[acyl-carrier protein] and tetradecanoyl-[acyl-carrier protein] decreased about 100fold and 30fold. The triple mutant M197R/R199H/T231K is a tetradecanoyl-[acyl-carrier protein] thioesterase
Umbellularia californica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
dodecanoyl-[acyl-carrier protein]
competitive with respect to tetradecanoyl-[acyl-carrier protein], wild type enzyme and mutant enzyme M197R/R199H/T123K
Umbellularia californica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0019
-
dodecanoyl-[acyl-carrier protein]
wild type enzyme
Umbellularia californica
0.0023
-
tetradecanoyl-[acyl-carrier protein]
triple mutant M197R/R199H/T231K
Umbellularia californica
0.0064
-
tetradecanoyl-[acyl-carrier protein]
wild type enzyme
Umbellularia californica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dodecanoyl-[acyl-carrier protein] + H2O
the C-terminal two-third of the protein is critical for the specificity
23907
Umbellularia californica
[acyl-carrier protein] + dodecanoate
-
-
-
-
tetradecanoyl-[acyl-carrier protein] + H2O
the C-terminal two-third of the protein is critical for the specificity
23907
Umbellularia californica
[acyl-carrier protein] + tetradecanoate
-
-
-
-
Other publictions for EC 3.1.2.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750616
Work
Lauric acid production in a g ...
Synechococcus sp. PCC 7002
Front. Bioeng. Biotechnol.
3
48
2015
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750666
Reynolds
Metabolic engineering of medi ...
Umbellularia californica
Front. Plant Sci.
6
164
2015
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1
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23908
Voelker
Plant acyl-ACP thioesterase: c ...
Umbellularia californica
Genet. Eng. (N.Y.)
18
111-133
1996
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1
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2
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1
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23909
Eccleston
-
Medium-chain fatty acid biosyn ...
Umbellularia californica
Planta
198
46-53
1996
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1
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23907
Yuan
Modification of the substrate ...
Umbellularia californica
Proc. Natl. Acad. Sci. USA
92
10639-10643
1995
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1
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1
3
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6
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23905
Pollard
A specific acyl-ACP thioestera ...
Umbellularia californica
Arch. Biochem. Biophys.
284
306-312
1991
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3
5
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5
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4
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23906
Davies
Developmental induction, purif ...
Umbellularia californica
Arch. Biochem. Biophys.
290
37-45
1991
5
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1
8
1
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5
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3
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1
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