BRENDA - Enzyme Database show
show all sequences of 3.1.2.21

Developmental induction, purification, and further characterization of 12:0-ACP thioesterase from immature cotyledons of Umbellularia californica

Davies, H.M.; Anderson, L.; Fan, C.; Hawkins, D.J.; Arch. Biochem. Biophys. 290, 37-45 (1991)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
CHAPS
stimulation
Umbellularia californica
cholate
slight stimulation at 0.1 and 0.01% w/v. Strong inhibition at 1% w/v
Umbellularia californica
octylglucoside
stimulation at 0.1 and 0.01% w/v. Strong inhibition at 1% w/v
Umbellularia californica
Triton X-100
stimulation
Umbellularia californica
Zwittergent 3-8
stimulation
Umbellularia californica
General Stability
General Stability
Organism
stable to repeated thawings
Umbellularia californica
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
1 mM, 50% inhibition
Umbellularia californica
cholate
slight stimulation at 0.1 and 0.01% w/v. Strong inhibition at 1% w/v
Umbellularia californica
Cu2+
1 mM, 100% inhibition
Umbellularia californica
diethyldicarbonate
1 mM, 97% inhibition
Umbellularia californica
Mg2+
1 mM, 50% inhibition
Umbellularia californica
Mn2+
1 mM, 50% inhibition
Umbellularia californica
octylglucoside
stimulation at 0.1 and 0.01% w/v. Strong inhibition at 1% w/v
Umbellularia californica
Zn2+
1 mM, 100% inhibition
Umbellularia californica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
0.003
dodecanoyl-[acyl-carrier protein]
-
Umbellularia californica
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
NaCl
optimal concentration: 20-40 mM
Umbellularia californica
phosphate
optimal concentration: 15-20 mM
Umbellularia californica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
1 * 34000, SDS-PAGE
Umbellularia californica
42000
-
gel filtration
Umbellularia californica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dodecanoyl-[acyl-carrier protein] + H2O
Umbellularia californica
enzyme plays a major role in medium-chain fatty acid production
[acyl-carrier protein] + dodecanoate
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Umbellularia californica
-
-
-
Purification (Commentary)
Commentary
Organism
-
Umbellularia californica
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cotyledon
immature
Umbellularia californica
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.17
-
-
Umbellularia californica
Storage Stability
Storage Stability
Organism
-70°C, stable
Umbellularia californica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dodecanoyl-[acyl-carrier protein] + H2O
-
23906
Umbellularia californica
[acyl-carrier protein] + dodecanoate
-
-
-
-
dodecanoyl-[acyl-carrier protein] + H2O
enzyme plays a major role in medium-chain fatty acid production
23906
Umbellularia californica
[acyl-carrier protein] + dodecanoate
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 34000, SDS-PAGE
Umbellularia californica
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1
-
Ca2+
-
Umbellularia californica
1
-
Mg2+
-
Umbellularia californica
1
-
Mn2+
-
Umbellularia californica
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
CHAPS
stimulation
Umbellularia californica
cholate
slight stimulation at 0.1 and 0.01% w/v. Strong inhibition at 1% w/v
Umbellularia californica
octylglucoside
stimulation at 0.1 and 0.01% w/v. Strong inhibition at 1% w/v
Umbellularia californica
Triton X-100
stimulation
Umbellularia californica
Zwittergent 3-8
stimulation
Umbellularia californica
General Stability (protein specific)
General Stability
Organism
stable to repeated thawings
Umbellularia californica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
1 mM, 50% inhibition
Umbellularia californica
cholate
slight stimulation at 0.1 and 0.01% w/v. Strong inhibition at 1% w/v
Umbellularia californica
Cu2+
1 mM, 100% inhibition
Umbellularia californica
diethyldicarbonate
1 mM, 97% inhibition
Umbellularia californica
Mg2+
1 mM, 50% inhibition
Umbellularia californica
Mn2+
1 mM, 50% inhibition
Umbellularia californica
octylglucoside
stimulation at 0.1 and 0.01% w/v. Strong inhibition at 1% w/v
Umbellularia californica
Zn2+
1 mM, 100% inhibition
Umbellularia californica
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1
-
Ca2+
-
Umbellularia californica
1
-
Mg2+
-
Umbellularia californica
1
-
Mn2+
-
Umbellularia californica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
0.003
dodecanoyl-[acyl-carrier protein]
-
Umbellularia californica
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
NaCl
optimal concentration: 20-40 mM
Umbellularia californica
phosphate
optimal concentration: 15-20 mM
Umbellularia californica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
1 * 34000, SDS-PAGE
Umbellularia californica
42000
-
gel filtration
Umbellularia californica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dodecanoyl-[acyl-carrier protein] + H2O
Umbellularia californica
enzyme plays a major role in medium-chain fatty acid production
[acyl-carrier protein] + dodecanoate
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Umbellularia californica
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cotyledon
immature
Umbellularia californica
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.17
-
-
Umbellularia californica
Storage Stability (protein specific)
Storage Stability
Organism
-70°C, stable
Umbellularia californica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dodecanoyl-[acyl-carrier protein] + H2O
-
23906
Umbellularia californica
[acyl-carrier protein] + dodecanoate
-
-
-
-
dodecanoyl-[acyl-carrier protein] + H2O
enzyme plays a major role in medium-chain fatty acid production
23906
Umbellularia californica
[acyl-carrier protein] + dodecanoate
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 34000, SDS-PAGE
Umbellularia californica
Other publictions for EC 3.1.2.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750616
Work
Lauric acid production in a g ...
Synechococcus sp. PCC 7002
Front. Bioeng. Biotechnol.
3
48
2015
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
750666
Reynolds
Metabolic engineering of medi ...
Umbellularia californica
Front. Plant Sci.
6
164
2015
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
23908
Voelker
Plant acyl-ACP thioesterase: c ...
Umbellularia californica
Genet. Eng. (N.Y.)
18
111-133
1996
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
23909
Eccleston
-
Medium-chain fatty acid biosyn ...
Umbellularia californica
Planta
198
46-53
1996
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
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-
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1
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-
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-
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1
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-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
23907
Yuan
Modification of the substrate ...
Umbellularia californica
Proc. Natl. Acad. Sci. USA
92
10639-10643
1995
-
-
1
-
1
-
1
3
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
23905
Pollard
A specific acyl-ACP thioestera ...
Umbellularia californica
Arch. Biochem. Biophys.
284
306-312
1991
-
-
-
-
-
-
3
5
-
-
-
-
-
3
-
-
1
-
-
1
-
1
4
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
2
5
-
-
-
-
-
-
-
1
-
1
-
1
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
23906
Davies
Developmental induction, purif ...
Umbellularia californica
Arch. Biochem. Biophys.
290
37-45
1991
5
-
-
-
-
1
8
1
-
2
2
1
-
1
-
-
1
-
-
1
1
1
2
1
-
-
-
-
-
-
-
-
3
-
-
5
-
-
-
-
-
1
-
8
3
1
-
2
2
1
-
-
-
1
-
1
1
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-