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Literature summary for 3.1.2.2 extracted from

  • Sabri, A.; Bare, G.; Jacques, P.; Jabrane, A.; Ongena, M.; Van Heugen, J.C.; Devreese, B.; Thonart, P.
    Influence of moderate temperatures on myristoyl-CoA metabolism and acyl-CoA thioesterase activity in the psychrophilic antarctic yeast Rhodotorula aurantiaca (2001), J. Biol. Chem., 276, 12691-12696.
    View publication on PubMed
Search for more literature for 3.1.2.2

Inhibitors

Inhibitors Comment Organism Structure
diisopropylfluorophosphate complete inhibition Buckleyzyma aurantiaca
myristoyl-CoA substrate inhibition, in vivo 28fold increased substrate concentration at 20°C leads to reduced enzyme activity Buckleyzyma aurantiaca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km at different temperatures Buckleyzyma aurantiaca
additional information
-
 additional information Km for myristoyl-CoA is 59fold higher at 20°C compared to 5°C Buckleyzyma aurantiaca
0.018
-
 myristoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
0.018
-
 tetradecanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
0.023
-
 palmitoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
0.023
-
 hexadecanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
0.024
-
 stearoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
0.024
-
 octadecanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
0.058
-
 palmitoleoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
0.058
-
 palmitoleoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
0.067
-
 decanoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
0.067
-
 decanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
0.118
-
 octanoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
0.118
-
 octanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 1 * 80000, SDS-PAGE Buckleyzyma aurantiaca
85000
-
 gel filtration Buckleyzyma aurantiaca

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Buckleyzyma aurantiaca extreme accumulation, 28fold increased compared to 0°C, of myristoyl-CoA in cells at nonpermissive temperature close to 20°C, causing growth arrest ?
-
 ?
myristoyl-CoA + H2O Buckleyzyma aurantiaca highly preferred substrate, temperature regulated activity, mechanism CoA + myristate
-
 ?
tetradecanoyl-CoA + H2O Buckleyzyma aurantiaca
-
 CoA + tetradecanoate
-
 ?

Organism

Organism UniProt Comment Textmining
Buckleyzyma aurantiaca
-
 psychrophilic antarctic yeast, strain A19
-
Buckleyzyma aurantiaca
-
 psychrophilic yeast, strain A19
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein highly glycosylated, 19% of the molecular weight is due to carbohydrates Buckleyzyma aurantiaca
glycoprotein 19% of the MW is formed by the glycosidic portion Buckleyzyma aurantiaca

Purification (Commentary)

Purification (Comment) Organism
351fold Buckleyzyma aurantiaca

Reaction

Reaction Comment Organism Reaction ID
palmitoyl-CoA + H2O = CoA + palmitate active site serine residue Buckleyzyma aurantiaca
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
108
-
 purified enzyme Buckleyzyma aurantiaca
108
-
 purified enzyme, substrate myristoyl-CoA Buckleyzyma aurantiaca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + H2O
-
 Buckleyzyma aurantiaca CoA + a carboxylate
-
 ?
decanoyl-CoA + H2O
-
 Buckleyzyma aurantiaca CoA + decanoate
-
 ?
hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Buckleyzyma aurantiaca CoA + hexadecanoate
-
 ?
additional information no activity with short-chain acyl-CoA substrates Buckleyzyma aurantiaca ?
-
 ?
additional information extreme accumulation, 28fold increased compared to 0°C, of myristoyl-CoA in cells at nonpermissive temperature close to 20°C, causing growth arrest Buckleyzyma aurantiaca ?
-
 ?
myristoyl-CoA + H2O highly preferred substrate Buckleyzyma aurantiaca CoA + myristate
-
 ?
myristoyl-CoA + H2O highly preferred substrate, temperature regulated activity, mechanism Buckleyzyma aurantiaca CoA + myristate
-
 ?
octadecanoyl-CoA + H2O i.e. stearoyl-CoA Buckleyzyma aurantiaca CoA + octadecanoate
-
 ?
octanoyl-CoA + H2O
-
 Buckleyzyma aurantiaca CoA + octanoate
-
 ?
palmitoleoyl-CoA + H2O
-
 Buckleyzyma aurantiaca CoA + palmitoleoate
-
 ?
palmitoyl-CoA + H2O
-
 Buckleyzyma aurantiaca CoA + palmitate
-
 ?
stearoyl-CoA + H2O
-
 Buckleyzyma aurantiaca CoA + stearate
-
 ?
tetradecanoyl-CoA + H2O
-
 Buckleyzyma aurantiaca CoA + tetradecanoate
-
 ?
tetradecanoyl-CoA + H2O specific for Buckleyzyma aurantiaca CoA + tetradecanoate
-
 ?
tetradecanoyl-CoA + H2O best substrate Buckleyzyma aurantiaca CoA + tetradecanoate
-
 ?
tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Buckleyzyma aurantiaca CoA + tetradecanoate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 80000, SDS-PAGE Buckleyzyma aurantiaca

Synonyms

Synonyms Comment Organism
long chain acyl-CoA thioesterase
-
 Buckleyzyma aurantiaca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5
-
 below, optimal growth temperature in vivo Buckleyzyma aurantiaca
45
-
-
 Buckleyzyma aurantiaca

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 organism is unable to grow at temperatures close to 20°C or above Buckleyzyma aurantiaca
additional information
-
 the strain is inable to grow at temperatures close to 20°C and higher Buckleyzyma aurantiaca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 60 min, pH 7.0, 82% remaining activity Buckleyzyma aurantiaca
40
-
 60 min, 82% remaining activity Buckleyzyma aurantiaca
50
-
 60 min, pH 7.0, 52% remaining activity Buckleyzyma aurantiaca
50
-
 60 min, 52% remaining activity Buckleyzyma aurantiaca
60
-
 60 min, pH 7.0, 3% remaining activity Buckleyzyma aurantiaca
60
-
 60 min, 3% remaining activity Buckleyzyma aurantiaca

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
106
-
 palmitoleoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
106
-
 palmitoleoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
121
-
 stearoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
121
-
 stearoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
136
-
 decanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
136
-
 decanoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
153
-
 palmitoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
153
-
 palmitoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
159
-
 octanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
159
-
 octanoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
183
-
 myristoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
183
-
 myristoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Buckleyzyma aurantiaca

pH Range

pH Minimum pH Maximum Comment Organism
5 10
-
 Buckleyzyma aurantiaca
5 10 active and stable Buckleyzyma aurantiaca

pI Value

Organism Comment pI Value Maximum pI Value
Buckleyzyma aurantiaca
-
-
 4.4
Buckleyzyma aurantiaca isoelectric focusing
-
 4.4