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Literature summary for 3.1.2.2 extracted from
- Purification and characterization of a long-chain acyl-CoA hydrolase from rat liver microsomes (1979), Biochim. Biophys. Acta, 574, 321-333.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Rattus norvegicus |  |
| Triton X-100 | - |
Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Rattus norvegicus | |
| KCl | - |
Rattus norvegicus | |
| Mg2+ | 0.5 mM gives 40% inhibition | Rattus norvegicus | |
| Mn2+ | 0.2 mM gives 60% inhibition | Rattus norvegicus | |
| N-ethylmaleimide | 0.001 mM gives 50% inhibition | Rattus norvegicus | |
| NaCl | - |
Rattus norvegicus | |
| p-hydroxymercuribenzoate | 0.005 mM gives 50% inhibition | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
hexadecanoyl-CoA | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
x * 35000, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| palmitoyl-CoA + H2O | Rattus norvegicus | - |
CoA + palmitate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.15 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C over 1 month | Rattus norvegicus |
| -80°C for several months without notable loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + H2O | long-chain fatty-acyl-CoA | Rattus norvegicus | CoA + a carboxylate | - |
? | |
| dodecanoyl-CoA + H2O | i.e. lauroyl-CoA | Rattus norvegicus | CoA + dodecanoate | - |
? | |
| hexadecanoyl-CoA + H2O | best substrate | Rattus norvegicus | CoA + hexadecanoate | - |
? | |
| hexadecanoyl-CoA + H2O | i.e. palmitoyl-CoA | Rattus norvegicus | CoA + hexadecanoate | - |
? | |
| octadecanoyl-CoA + H2O | i.e. stearoyl-CoA | Rattus norvegicus | CoA + octadecanoate | - |
? | |
| oleoyl-CoA + H2O | - |
Rattus norvegicus | CoA + oleate | - |
? | |
| palmitoyl-CoA + H2O | - |
Rattus norvegicus | CoA + palmitate | - |
? | |
| tetradecanoyl-CoA + H2O | i.e. myristoyl-CoA | Rattus norvegicus | CoA + tetradecanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, SDS-PAGE | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
- |
Rattus norvegicus |
| 7.5 | 8 | - |
Rattus norvegicus |
| 7.8 | 8.2 | - |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8.2 | - |
Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
N-ethylmaleimide | - |
Rattus norvegicus | |
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