Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | competitive inhibition | Rattus norvegicus | |
butyryl-CoA | competitive | Rattus norvegicus | |
CoA | competitive inhibition | Rattus norvegicus | |
decanoyl-CoA | noncompetitive inhibition | Rattus norvegicus | |
glutaryl-CoA | competitive inhibition | Rattus norvegicus | |
heptanoyl-CoA | competitive inhibition | Rattus norvegicus | |
hexanoyl-CoA | competitive inhibition | Rattus norvegicus | |
malonyl-CoA | competitive inhibition | Rattus norvegicus | |
nonanoyl-CoA | noncompetitive inhibition | Rattus norvegicus | |
octanoyl-CoA | noncompetitive inhibition | Rattus norvegicus | |
palmitoyl-CoA | noncompetitive inhibition | Rattus norvegicus | |
pentanoyl-CoA | competitive inhibition | Rattus norvegicus | |
propionyl-CoA | competitive inhibition | Rattus norvegicus | |
succinyl-CoA | competitive inhibition | Rattus norvegicus | |
tridecanoyl-CoA | noncompetitive inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
malonyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
0.7 | - |
(S)-methylmalonyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
1.4 | - |
DL-methylmalonyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
1.9 | - |
propionyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
14 | - |
acetyl-CoA | pH 6.0, 30°C | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-methylmalonyl-CoA + H2O | Rattus norvegicus | - |
methylmalonate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.41 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme stable for at least 1 month | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-methylmalonyl-CoA + H2O | - |
Rattus norvegicus | methylmalonate + CoA | - |
? | |
(S)-methylmalonyl-CoA + H2O | not active on the L-isomer | Rattus norvegicus | methylmalonate + CoA | - |
? | |
acetyl-CoA + H2O | - |
Rattus norvegicus | CoA + acetate | - |
? | |
DL-methylmalonyl-CoA + H2O | - |
Rattus norvegicus | CoA + methylmalonate | - |
? | |
malonyl-CoA + H2O | 16% of the activity with (S)-methylmalonyl-CoA | Rattus norvegicus | malonate + CoA | - |
? | |
additional information | acetyl-CoA 1% of the activity with (S)-methylmalonyl-CoA , succinyl-CoA and palmitoyl-CoA hydrolyzed at less than 1% of the activity with (S)-methylmalonyl-CoA | Rattus norvegicus | ? | - |
? | |
propionyl-CoA + H2O | 3% of the activity with (S)-methylmalonyl-CoA | Rattus norvegicus | propionate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, SDS-PAGE, in absence and presence of 1% 2-mercaptoethanol | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23.3 | - |
(S)-methylmalonyl-CoA | pH 6.0, 30°C | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
50% activity at pH 5.3 and pH 7.1 | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
palmitoyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
0.04 | - |
tridecanoyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
0.05 | - |
decanoyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
0.2 | - |
nonanoyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
0.5 | - |
octanoyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
0.6 | - |
malonyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
1.4 | - |
CoA | pH 6.0, 30°C | Rattus norvegicus | |
1.5 | - |
succinyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
1.5 | - |
heptanoyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
1.7 | - |
hexanoyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
2.6 | - |
butyryl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
2.6 | - |
valeryl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
3 | - |
propionyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
4.1 | - |
acetyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
4.9 | - |
glutaryl-CoA | pH 6.0, 30°C | Rattus norvegicus |