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Literature summary for 3.1.2.17 extracted from

  • Kovachy, R.J.; Copley, S.D.; Allen, R.H.
    Recognition, isolation, and characterization of rat liver D-methylmalonyl coenzyme A hydrolase (1983), J. Biol. Chem., 258, 11415-11421.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA competitive inhibition Rattus norvegicus
butyryl-CoA competitive Rattus norvegicus
CoA competitive inhibition Rattus norvegicus
decanoyl-CoA noncompetitive inhibition Rattus norvegicus
glutaryl-CoA competitive inhibition Rattus norvegicus
heptanoyl-CoA competitive inhibition Rattus norvegicus
hexanoyl-CoA competitive inhibition Rattus norvegicus
malonyl-CoA competitive inhibition Rattus norvegicus
nonanoyl-CoA noncompetitive inhibition Rattus norvegicus
octanoyl-CoA noncompetitive inhibition Rattus norvegicus
palmitoyl-CoA noncompetitive inhibition Rattus norvegicus
pentanoyl-CoA competitive inhibition Rattus norvegicus
propionyl-CoA competitive inhibition Rattus norvegicus
succinyl-CoA competitive inhibition Rattus norvegicus
tridecanoyl-CoA noncompetitive inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
malonyl-CoA pH 6.0, 30°C Rattus norvegicus
0.7
-
(S)-methylmalonyl-CoA pH 6.0, 30°C Rattus norvegicus
1.4
-
DL-methylmalonyl-CoA pH 6.0, 30°C Rattus norvegicus
1.9
-
propionyl-CoA pH 6.0, 30°C Rattus norvegicus
14
-
acetyl-CoA pH 6.0, 30°C Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-methylmalonyl-CoA + H2O Rattus norvegicus
-
methylmalonate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.41
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, purified enzyme stable for at least 1 month Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-methylmalonyl-CoA + H2O
-
Rattus norvegicus methylmalonate + CoA
-
?
(S)-methylmalonyl-CoA + H2O not active on the L-isomer Rattus norvegicus methylmalonate + CoA
-
?
acetyl-CoA + H2O
-
Rattus norvegicus CoA + acetate
-
?
DL-methylmalonyl-CoA + H2O
-
Rattus norvegicus CoA + methylmalonate
-
?
malonyl-CoA + H2O 16% of the activity with (S)-methylmalonyl-CoA Rattus norvegicus malonate + CoA
-
?
additional information acetyl-CoA 1% of the activity with (S)-methylmalonyl-CoA , succinyl-CoA and palmitoyl-CoA hydrolyzed at less than 1% of the activity with (S)-methylmalonyl-CoA Rattus norvegicus ?
-
?
propionyl-CoA + H2O 3% of the activity with (S)-methylmalonyl-CoA Rattus norvegicus propionate + CoA
-
?

Subunits

Subunits Comment Organism
monomer 1 * 35000, SDS-PAGE, in absence and presence of 1% 2-mercaptoethanol Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23.3
-
(S)-methylmalonyl-CoA pH 6.0, 30°C Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
50% activity at pH 5.3 and pH 7.1 Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.01
-
palmitoyl-CoA pH 6.0, 30°C Rattus norvegicus
0.04
-
tridecanoyl-CoA pH 6.0, 30°C Rattus norvegicus
0.05
-
decanoyl-CoA pH 6.0, 30°C Rattus norvegicus
0.2
-
nonanoyl-CoA pH 6.0, 30°C Rattus norvegicus
0.5
-
octanoyl-CoA pH 6.0, 30°C Rattus norvegicus
0.6
-
malonyl-CoA pH 6.0, 30°C Rattus norvegicus
1.4
-
CoA pH 6.0, 30°C Rattus norvegicus
1.5
-
succinyl-CoA pH 6.0, 30°C Rattus norvegicus
1.5
-
heptanoyl-CoA pH 6.0, 30°C Rattus norvegicus
1.7
-
hexanoyl-CoA pH 6.0, 30°C Rattus norvegicus
2.6
-
butyryl-CoA pH 6.0, 30°C Rattus norvegicus
2.6
-
valeryl-CoA pH 6.0, 30°C Rattus norvegicus
3
-
propionyl-CoA pH 6.0, 30°C Rattus norvegicus
4.1
-
acetyl-CoA pH 6.0, 30°C Rattus norvegicus
4.9
-
glutaryl-CoA pH 6.0, 30°C Rattus norvegicus