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Literature summary for 3.1.2.16 extracted from
- Citrate lyase deacetylase of Rhodopseudomonas gelatinosa. Isolation of the enzyme and studies on the inhibition by L-glutamate (1980), Eur. J. Biochem., 111, 461-471.
General Stability
| General Stability | Organism |
|---|---|
| loses activity upon repeated freezing and thawing | Rubrivivax gelatinosus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-glutamyl-glutamate | 10 mM, 70% inhibition | Rubrivivax gelatinosus |  |
| D-glutamate | 10 mM, 54% inhibition | Rubrivivax gelatinosus | |
| gamma-glutamyl-glutamate | 10 mM, 70% inhibition | Rubrivivax gelatinosus | |
| L-aspartate | 10 mM, 60% inhibition | Rubrivivax gelatinosus | |
| L-glutamate | linear mixed type of inhibition | Rubrivivax gelatinosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 7300 | - |
2 * 7300, SDS-PAGE | Rubrivivax gelatinosus |
| 14300 | - |
gel filtration | Rubrivivax gelatinosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [citrate (pro-3S)-lyase](acetyl form) + H2O | Rubrivivax gelatinosus | regulates the activity of citrate layse by covalent modification | [citrate (pro-3S)-lyase](thiol form) + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| no activity in Enterobacter aerogenes | - |
- |
- |
| no activity in Escherichia coli | - |
- |
- |
| no activity in Streptococcus diacetilactis | - |
- |
- |
| Rubrivivax gelatinosus | - |
DSM 149 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rubrivivax gelatinosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activity 1000 U/mg | Rubrivivax gelatinosus |
| additional information | - |
specific activity 15.00 U/mg | Rubrivivax gelatinosus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, partly purified enzyme retains full activity for at least 1 year | Rubrivivax gelatinosus |
| -20°C, purified enzyme loses 20% of activity within 1 year during storage | Rubrivivax gelatinosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [citrate (pro-3S)-lyase](acetyl form) + H2O | - |
Rubrivivax gelatinosus | [citrate (pro-3S)-lyase](thiol form) + acetate | - |
? | |
| [citrate (pro-3S)-lyase](acetyl form) + H2O | regulates the activity of citrate layse by covalent modification | Rubrivivax gelatinosus | [citrate (pro-3S)-lyase](thiol form) + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 7300, SDS-PAGE | Rubrivivax gelatinosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetyl-S-(acyl-carrier protein) enzyme thioester hydrolase (acetate) | - |
Rubrivivax gelatinosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
half-life 20 min, effectively protected against heat inactivation by 100 mM L-glutamate, half-life 30 min | Rubrivivax gelatinosus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
L-glutamate | pH 7.5, 30°C | Rubrivivax gelatinosus | |
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