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Literature summary for 3.1.2.14 extracted from

  • Yuan, L.; Nelson, B.A.; Caryl, G.
    The catalytic cysteine and histidine in the plant acyl-acyl carrier protein thioesterases (1996), J. Biol. Chem., 271, 3417-3419.
    View publication on PubMed
Search for more literature for 3.1.2.14

Cloned(Commentary)

Cloned (Comment) Organism
expression of UcFatB1 and mutants in Escherichia coli Umbellularia californica

Protein Variants

Protein Variants Comment Organism
C320A no activity Umbellularia californica
C320S slightly reduced activity Umbellularia californica
H140A reduced activity Umbellularia californica
H140R reduced activity Umbellularia californica
H285A no activity Umbellularia californica
H285R reduced activity Umbellularia californica
H345A reduced activity Umbellularia californica
H345R reduced activity Umbellularia californica

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
 Umbellularia californica
diethyldicarbonate
-
 Umbellularia californica
iodoacetamide
-
 Umbellularia californica
additional information no inhibition by phenylmethylsulfonylfluoride Umbellularia californica

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Brassica napus
-
-
-
Carthamus tinctorius
-
-
-
Umbellularia californica
-
 bay, UcFatB1
-

Purification (Commentary)

Purification (Comment) Organism
Uc FatB1 and mutants Umbellularia californica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lauroyl-[acyl-carrier protein] + H2O
-
 Umbellularia californica lauric acid + acyl-carrier protein
-
 ?