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Literature summary for 3.1.2.14 extracted from

  • Hernandez Lozada, N.J.; Lai, R.Y.; Simmons, T.R.; Thomas, K.A.; Chowdhury, R.; Maranas, C.D.; Pfleger, B.F.
    Highly active C8-acyl-ACP thioesterase variant isolated by a synthetic selection strategy (2018), ACS Synth. Biol., 7, 2205-2215 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DELTAlipB cells Cuphea palustris

Protein Variants

Protein Variants Comment Organism
A59S/K296R the mutant exhibits 4fold higher enzymatic activities than the wild type enzyme in vivo Cuphea palustris
DELTAA54/N28S/I65M the mutant exhibits much higher enzymatic activities than the wild type enzyme in vivo Cuphea palustris
H224A catalytically inactive Cuphea palustris
K15E/S207T the mutant exhibits wild type activity Cuphea palustris
L251M/L265I the mutant exhibits wild type activity Cuphea palustris
M29T/T117S/Q163L the mutant exhibits wild type activity Cuphea palustris
R261S the mutant exhibits higher enzymatic activities than the wild type enzyme in vivo Cuphea palustris
W17R/T204S the mutant exhibits higher enzymatic activities than the wild type enzyme in vivo Cuphea palustris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00592
-
octanoyl-[acyl-carrier protein] at pH 7.4, temperature not specified in the publication Cuphea palustris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
octanoyl-[acyl-carrier protein] + H2O Cuphea palustris specific substrate [acyl-carrier protein] + octanoate
-
?

Organism

Organism UniProt Comment Textmining
Cuphea palustris Q39554
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Cuphea palustris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
octanoyl-[acyl-carrier protein] + H2O specific substrate Cuphea palustris [acyl-carrier protein] + octanoate
-
?

Synonyms

Synonyms Comment Organism
C8-acyl-ACP thioesterase
-
Cuphea palustris
FatB1 thioesterase
-
Cuphea palustris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14
-
octanoyl-[acyl-carrier protein] at pH 7.4, temperature not specified in the publication Cuphea palustris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
193.3
-
octanoyl-[acyl-carrier protein] at pH 7.4, temperature not specified in the publication Cuphea palustris