Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DELTAlipB cells | Cuphea palustris |
Protein Variants | Comment | Organism |
---|---|---|
A59S/K296R | the mutant exhibits 4fold higher enzymatic activities than the wild type enzyme in vivo | Cuphea palustris |
DELTAA54/N28S/I65M | the mutant exhibits much higher enzymatic activities than the wild type enzyme in vivo | Cuphea palustris |
H224A | catalytically inactive | Cuphea palustris |
K15E/S207T | the mutant exhibits wild type activity | Cuphea palustris |
L251M/L265I | the mutant exhibits wild type activity | Cuphea palustris |
M29T/T117S/Q163L | the mutant exhibits wild type activity | Cuphea palustris |
R261S | the mutant exhibits higher enzymatic activities than the wild type enzyme in vivo | Cuphea palustris |
W17R/T204S | the mutant exhibits higher enzymatic activities than the wild type enzyme in vivo | Cuphea palustris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00592 | - |
octanoyl-[acyl-carrier protein] | at pH 7.4, temperature not specified in the publication | Cuphea palustris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
octanoyl-[acyl-carrier protein] + H2O | Cuphea palustris | specific substrate | [acyl-carrier protein] + octanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cuphea palustris | Q39554 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Cuphea palustris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
octanoyl-[acyl-carrier protein] + H2O | specific substrate | Cuphea palustris | [acyl-carrier protein] + octanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C8-acyl-ACP thioesterase | - |
Cuphea palustris |
FatB1 thioesterase | - |
Cuphea palustris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.14 | - |
octanoyl-[acyl-carrier protein] | at pH 7.4, temperature not specified in the publication | Cuphea palustris |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
193.3 | - |
octanoyl-[acyl-carrier protein] | at pH 7.4, temperature not specified in the publication | Cuphea palustris |