Application | Comment | Organism |
---|---|---|
industry | contribution of FatA to the synthesis of castor oil | Ricinus communis |
industry | contribution of FatB to the synthesis of castor oil | Ricinus communis |
Cloned (Comment) | Organism |
---|---|
PCR fragments cloned into the pMBL-T vector, subcloned into the BamHI-HindIII or SacI-KpnI sites of the pQE-80L vector. Resulting construct pQERcFatA overexpressed in Escherichia coli XL1-Blue | Ricinus communis |
PCR fragments cloned into the pMBL-T vector, subcloned into the BamHI-HindIII or SacI-KpnI sites of the pQE-80L vector. Resulting construct pQERcFatB overexpressed in Escherichia coli XL1-Blue | Ricinus communis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00035 | - |
palmitoleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
0.00042 | - |
oleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
0.00054 | - |
palmitoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
0.00054 | - |
stearoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
0.00056 | - |
palmitoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
0.00067 | - |
oleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
0.00086 | - |
palmitoleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
0.00088 | - |
stearoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36800 | - |
recombinant histidine-tagged FatB, gel filtration | Ricinus communis |
37520 | - |
recombinant histidine-tagged FatA, gel filtration | Ricinus communis |
42200 | - |
FatA open reading frames, calculated from sequence | Ricinus communis |
46500 | - |
FatB open reading frames, calculated from sequence | Ricinus communis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ricinus communis | A9XK92 | - |
- |
Ricinus communis | A9XUG5 | - |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity in a single step by gel filtration | Ricinus communis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Ricinus communis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | FatA enzyme displays low levels of activity on saturated stearoyl- and palmitoyl-[acyl-carrier protein] derivatives | Ricinus communis | ? | - |
? | |
oleoyl-[acyl-carrier protein] + H2O | high efficiency of FatB | Ricinus communis | oleic acid + acyl-carrier protein | - |
? | |
oleoyl-[acyl-carrier protein] + H2O | maximum catalytic efficiency of FatA | Ricinus communis | oleic acid + acyl-carrier protein | - |
? | |
palmitoleoyl-[acyl-carrier protein] + H2O | high catalytic efficiency of FatA | Ricinus communis | palmitoleate + acyl-carrier protein | - |
? | |
palmitoleoyl-[acyl-carrier protein] + H2O | high efficiency of FatB | Ricinus communis | palmitoleate + acyl-carrier protein | - |
? | |
palmitoyl-[acyl-carrier protein] + H2O | - |
Ricinus communis | palmitate + acyl-carrier protein | - |
? | |
stearoyl-[acyl-carrier protein] + H2O | - |
Ricinus communis | stearate + acyl-carrier protein | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42200, FatA open reading frames, calculated from sequence | Ricinus communis |
? | x * 46500, FatA open reading frames, calculated from sequence | Ricinus communis |
Synonyms | Comment | Organism |
---|---|---|
acyl-ACP thioesterase | - |
Ricinus communis |
acyl-acyl carrier protein (ACP) thioesterase | - |
Ricinus communis |
acylACP thioesterase | - |
Ricinus communis |
acylacyl carrier protein (ACP) thioesterase | - |
Ricinus communis |
FatA | - |
Ricinus communis |
FatB | - |
Ricinus communis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
palmitoleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
0.38 | - |
stearoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
0.41 | - |
palmitoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
0.46 | - |
palmitoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
0.56 | - |
stearoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
0.67 | - |
oleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
1.62 | - |
palmitoleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
7.99 | - |
oleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ricinus communis | calculated from sequence | - |
6.7 |
Ricinus communis | calculated from sequence | - |
7 |
Organism | Comment | Expression |
---|---|---|
Ricinus communis | transcript accumulation profile is temporally regulated during embryo development. Displays maximum level of transcription in the period of maximum oil accumulation | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
250 | - |
palmitoleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
440 | - |
stearoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
760 | - |
palmitoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
830 | - |
palmitoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
990 | - |
oleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
1030 | - |
stearoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
4630 | - |
palmitoleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
19030 | - |
oleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis |