Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.2.14 extracted from

  • Bhattacharjee, A.; Ghosh, S.K.; Ghosh, D.; Ghosh, S.; Maiti, M.K.; Sen, S.K.
    Identification of a heat-stable palmitoyl/oleoyl specific acyl-acyl carrier protein thioesterase in developing seeds of Madhuca butyracea (2002), Plant Sci., 163, 791-800.
No PubMed abstract available
Search for more literature for 3.1.2.14

General Stability

General Stability Organism
glycerol, 25%, is required for stability during purification for the enzyme form PO-FAT Diploknema butyracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0034
-
 stearoyl-[acyl-carrier protein] enzyme form PO-FAT, pH 9.0, 37°C Diploknema butyracea
0.0036
-
 palmitoyl-[acyl-carrier protein] enzyme form PO-FAT, pH 9.0, 37°C Diploknema butyracea
0.008
-
 oleoyl-[acyl-carrier protein] enzyme form PO-FAT, pH 9.0, 37°C Diploknema butyracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration Diploknema butyracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oleoyl-[acyl-carrier protein] + H2O Diploknema butyracea
-
 oleic acid + acyl-carrier protein
-
 ?
palmitoyl-[acyl-carrier protein] + H2O Diploknema butyracea
-
 palmitic acid + acyl-carrier protein
-
 ?

Organism

Organism UniProt Comment Textmining
Diploknema butyracea
-
 i.e. Brassica butyracea, 2 enzyme forms: a palmitoyl-/oleoyl-[acyl carrier protein] specific and a oleoyl-[acyl carrier protein] specific one, i.e. PO-FAT and LC-FAT, respectively
-

Purification (Commentary)

Purification (Comment) Organism
enzyme form PO-FAT, 499fold Diploknema butyracea

Source Tissue

Source Tissue Comment Organism Textmining
flower bud
-
 Diploknema butyracea
-
leaf young Diploknema butyracea
-
seed developing, period from 45 to 93 days after flowering Diploknema butyracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Diploknema butyracea
0.69
-
 seed 65 days after flowering, enzyme form PO-FAT Diploknema butyracea
192
-
 purified enzyme form PO-FAT Diploknema butyracea

Storage Stability

Storage Stability Organism
-20°C, enzyme form PO-FAT, ammonium sulfate pellet, stable Diploknema butyracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oleoyl-[acyl-carrier protein] + H2O
-
 Diploknema butyracea oleic acid + acyl-carrier protein
-
 ?
oleoyl-[acyl-carrier protein] + H2O preferred substrate of the oleoyl-[acyl carrier protein] specific enzyme form LC-FAT Diploknema butyracea oleic acid + acyl-carrier protein
-
 ?
palmitoyl-[acyl-carrier protein] + H2O
-
 Diploknema butyracea palmitic acid + acyl-carrier protein
-
 ?
palmitoyl-[acyl-carrier protein] + H2O preferred substrate of the palmitoyl-/oleoyl-[acyl carrier protein] specific enzyme form PO-FAT Diploknema butyracea palmitic acid + acyl-carrier protein
-
 ?
stearoyl-[acyl-carrier protein] + H2O
-
 Diploknema butyracea stearic acid + acyl-carrier protein
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 42500-44000, SDS-PAGE Diploknema butyracea

Synonyms

Synonyms Comment Organism
12:0-ACP thioesterase
-
 Diploknema butyracea
12:0-acyl-carrier protein thioesterase
-
 Diploknema butyracea
14:0-ACP thioesterase
-
 Diploknema butyracea
14:0-acyl-carrier protein thioesterase
-
 Diploknema butyracea
16:0-ACP thioesterase
-
 Diploknema butyracea
16:0-acyl-carrier protein thioesterase
-
 Diploknema butyracea
18:0-ACP thioesterase
-
 Diploknema butyracea
18:0-acyl-carrier protein thioesterase
-
 Diploknema butyracea
acyl-ACP-hydrolase
-
 Diploknema butyracea
acyl-acyl carrier protein hydrolase
-
 Diploknema butyracea
acyl-acyl carrier protein thioesterase
-
 Diploknema butyracea
acyl-[acyl-carrier-protein] hydrolase
-
 Diploknema butyracea
AH
-
 Diploknema butyracea
BTE
-
 Diploknema butyracea
C12:0 ACP TE
-
 Diploknema butyracea
C18:1-ACP thioesterase
-
 Diploknema butyracea
Deubiquitinating enzyme 14
-
 Diploknema butyracea
FAT
-
 Diploknema butyracea
fatty acyl-ACP thioesterase
-
 Diploknema butyracea
hydrolase, oleoyl-[acyl carrier protein]
-
 Diploknema butyracea
LC-FAT oleoyl-[acyl carrier protein] specific Diploknema butyracea
oleoyl-ACP thioesterase
-
 Diploknema butyracea
oleoyl-acyl carrier protein thioesterase
-
 Diploknema butyracea
OTE
-
 Diploknema butyracea
PATE
-
 Diploknema butyracea
PO-FAT palmitoyl-/oleoyl-[acyl carrier protein] specific Diploknema butyracea
STE
-
 Diploknema butyracea
Ubiquitin thiolesterase 14
-
 Diploknema butyracea
Ubiquitin-specific processing protease 14
-
 Diploknema butyracea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Diploknema butyracea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 15 min, enzyme form LC-FAT is mostly inactivated, while enzyme form PO-FAT is activated by 15-20% Diploknema butyracea
70
-
 15 min, enzyme form PO-FAT is heat-stable, after 45 min the enzyme retains 30% of the original activity Diploknema butyracea
80
-
 15 min, 30% remaining activity Diploknema butyracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 enzyme form PO-FAT Diploknema butyracea

pI Value

Organism Comment pI Value Maximum pI Value
Diploknema butyracea enzyme form LC-FAT, chromatofocusing
-
 8.2
Diploknema butyracea enzyme form PO-FAT, chromatofocusing
-
 9.7