BRENDA - Enzyme Database
show all sequences of 3.1.2.12

Crystal structure of an S-formylglutathione hydrolase from Pseudoalteromonas haloplanktis TAC125

Alterio, V.; Aurilia, V.; Romanelli, A.; Parracino, A.; Saviano, M.; D'Auria, S.; De Simone, G.; Biopolymers 93, 669-677 (2010)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
N-terminus His6-S-tagged protein from pET28a construct overexpressed in Escherichia coli strain BL21(DE3) under the control of the T7 RNA polymerase transcription system
Pseudoalteromonas haloplanktis
Crystallization (Commentary)
Crystallization (Commentary)
Organism
by the hanging drop vapor diffusion technique, to 2.2 A resolution. Crystals belong to space group P212121 with unit cell dimensions of a = 49.49 A, b = 129.75 A, c = 152.67 A, with 4 molecules per asymmetric unit
Pseudoalteromonas haloplanktis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
2 * 30000, SDS-PAGE
Pseudoalteromonas haloplanktis
60000
-
gel filtration
Pseudoalteromonas haloplanktis
Organism
Organism
UniProt
Commentary
Textmining
Pseudoalteromonas haloplanktis
-
TAC125
-
Pseudoalteromonas haloplanktis TAC 125
-
TAC125
-
Purification (Commentary)
Purification (Commentary)
Organism
to homogeneity by a single-step Ni2+ affinity chromatography
Pseudoalteromonas haloplanktis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-nitrophenyl thioacetate + H2O
substrate with small acyl moiety
707759
Pseudoalteromonas haloplanktis
4-nitrophenol + thioacetate
-
-
-
?
4-nitrophenyl thioacetate + H2O
substrate with small acyl moiety
707759
Pseudoalteromonas haloplanktis TAC 125
4-nitrophenol + thioacetate
-
-
-
?
additional information
PhEst contains a catalytic triad formed by residues Ser147, Asp225 and His258. It shows no activity toward substrates with bulky acyl groups such as S-lactoylglutathione. Has a very narrow acyl-binding pocket in a typical alpha/beta-hydrolase fold
707759
Pseudoalteromonas haloplanktis
?
-
-
-
-
additional information
PhEst contains a catalytic triad formed by residues Ser147, Asp225 and His258. It shows no activity toward substrates with bulky acyl groups such as S-lactoylglutathione. Has a very narrow acyl-binding pocket in a typical alpha/beta-hydrolase fold
707759
Pseudoalteromonas haloplanktis TAC 125
?
-
-
-
-
S-acetylglutathione + H2O
substrate with small acyl moiety, high activity
707759
Pseudoalteromonas haloplanktis
glutathione + acetate
-
-
-
?
S-acetylglutathione + H2O
substrate with small acyl moiety, high activity
707759
Pseudoalteromonas haloplanktis TAC 125
glutathione + acetate
-
-
-
?
S-formylglutathione + H2O
substrate with small acyl moiety, lower activity than compared to S-acetylglutathione
707759
Pseudoalteromonas haloplanktis
glutathione + formate
-
-
-
?
S-formylglutathione + H2O
substrate with small acyl moiety, lower activity than compared to S-acetylglutathione
707759
Pseudoalteromonas haloplanktis TAC 125
glutathione + formate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 30000, SDS-PAGE
Pseudoalteromonas haloplanktis
Synonyms
Synonyms
Commentary
Organism
FGH
-
Pseudoalteromonas haloplanktis
PhEst
-
Pseudoalteromonas haloplanktis
S-formylglutathione hydrolase
-
Pseudoalteromonas haloplanktis
Cloned(Commentary) (protein specific)
Commentary
Organism
N-terminus His6-S-tagged protein from pET28a construct overexpressed in Escherichia coli strain BL21(DE3) under the control of the T7 RNA polymerase transcription system
Pseudoalteromonas haloplanktis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
by the hanging drop vapor diffusion technique, to 2.2 A resolution. Crystals belong to space group P212121 with unit cell dimensions of a = 49.49 A, b = 129.75 A, c = 152.67 A, with 4 molecules per asymmetric unit
Pseudoalteromonas haloplanktis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
2 * 30000, SDS-PAGE
Pseudoalteromonas haloplanktis
60000
-
gel filtration
Pseudoalteromonas haloplanktis
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity by a single-step Ni2+ affinity chromatography
Pseudoalteromonas haloplanktis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-nitrophenyl thioacetate + H2O
substrate with small acyl moiety
707759
Pseudoalteromonas haloplanktis
4-nitrophenol + thioacetate
-
-
-
?
4-nitrophenyl thioacetate + H2O
substrate with small acyl moiety
707759
Pseudoalteromonas haloplanktis TAC 125
4-nitrophenol + thioacetate
-
-
-
?
additional information
PhEst contains a catalytic triad formed by residues Ser147, Asp225 and His258. It shows no activity toward substrates with bulky acyl groups such as S-lactoylglutathione. Has a very narrow acyl-binding pocket in a typical alpha/beta-hydrolase fold
707759
Pseudoalteromonas haloplanktis
?
-
-
-
-
additional information
PhEst contains a catalytic triad formed by residues Ser147, Asp225 and His258. It shows no activity toward substrates with bulky acyl groups such as S-lactoylglutathione. Has a very narrow acyl-binding pocket in a typical alpha/beta-hydrolase fold
707759
Pseudoalteromonas haloplanktis TAC 125
?
-
-
-
-
S-acetylglutathione + H2O
substrate with small acyl moiety, high activity
707759
Pseudoalteromonas haloplanktis
glutathione + acetate
-
-
-
?
S-acetylglutathione + H2O
substrate with small acyl moiety, high activity
707759
Pseudoalteromonas haloplanktis TAC 125
glutathione + acetate
-
-
-
?
S-formylglutathione + H2O
substrate with small acyl moiety, lower activity than compared to S-acetylglutathione
707759
Pseudoalteromonas haloplanktis
glutathione + formate
-
-
-
?
S-formylglutathione + H2O
substrate with small acyl moiety, lower activity than compared to S-acetylglutathione
707759
Pseudoalteromonas haloplanktis TAC 125
glutathione + formate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 30000, SDS-PAGE
Pseudoalteromonas haloplanktis
Other publictions for EC 3.1.2.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751775
Xu
Regulation of S-formylglutath ...
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2
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2
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4
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2
2
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2
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2
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751579
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Esterase D enhances type I in ...
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75
112-121
2016
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1
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1
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751889
Huetten
Activity profiling reveals ch ...
Arabidopsis thaliana
Plant Physiol. Biochem.
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2015
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7
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1
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1
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731220
Legler
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528
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9
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15
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3
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9
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2
3
13
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2
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1
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15
1
-
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-
-
-
15
15
715774
Parracino
Towards nanoscale biomedical d ...
Pseudoalteromonas haloplanktis, Pseudoalteromonas haloplanktis TAC 125
J. Fluoresc.
21
663-672
2011
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1
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1
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11
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1
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2
1
1
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1
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1
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1
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1
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1
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2
1
-
-
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-
1
-
-
1
-
-
-
-
-
-
707759
Alterio
Crystal structure of an S-form ...
Pseudoalteromonas haloplanktis, Pseudoalteromonas haloplanktis TAC 125
Biopolymers
93
669-677
2010
-
-
1
1
-
-
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2
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11
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1
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8
1
3
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1
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1
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8
1
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716804
Liu
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Cloning, screening and charact ...
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Process Biochem.
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1
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1
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691781
Tuin
Activity-based protein profili ...
Homo sapiens
Chem. Res. Toxicol.
22
683-689
2009
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-
-
-
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2
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1
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1
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3
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1
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2
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694178
Kaschani
Diversity of serine hydrolase ...
Arabidopsis thaliana
Mol. Cell. Proteomics
8
1082-1093
2009
1
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6
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1
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1
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710495
van Straaten
The structure of a putative S- ...
Agrobacterium tumefaciens
Protein Sci.
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2196-2202
2009
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1
1
2
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1
6
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1
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7
1
3
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6
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6
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7
1
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6
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6
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691005
Legler
Structural characterization an ...
Saccharomyces cerevisiae
Biochemistry
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11
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24
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3
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3
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5
2
2
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24
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1
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11
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3
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24
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3
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5
2
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24
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682300
Cummins
Structure activity studies wit ...
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14
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14
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Cummins
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19
1
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1
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9
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17
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1
1
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19
1
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666299
Stover
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Phaeodactylum tricornutum, Sterkiella histriomuscorum, Tetrahymena thermophila, Thalassiosira pseudonana
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649219
McAuley
Purification, crystallization ...
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1
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653164
Yurimoto
Physiological role of S-formyl ...
Candida boidinii
Microbiology
149
1971-1979
2003
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1
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1
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2
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1
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4
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2
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1
1
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1
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1
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1
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1
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2
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1
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1
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649455
Kordic
Cloning and characterization o ...
Arabidopsis thaliana
Arch. Biochem. Biophys.
399
232-238
2002
1
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1
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4
7
-
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2
1
-
5
-
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1
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3
7
-
8
1
1
1
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1
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2
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1
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1
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1
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4
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7
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2
1
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1
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3
7
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8
1
1
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1
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2
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1
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-
-
653549
Haslam
-
Cloning and characterisation o ...
Arabidopsis thaliana
Plant Physiol. Biochem.
40
281-288
2002
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-
1
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2
2
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1
1
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1
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1
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5
1
1
1
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1
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1
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-
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-
-
2
-
2
-
-
1
1
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-
-
-
-
-
1
-
5
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
80990
Degrassi
Purification and properties of ...
Saccharomyces cerevisiae
Appl. Environ. Microbiol.
65
3470-3472
1999
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-
-
-
-
-
-
-
2
-
1
1
-
2
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-
-
-
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1
-
6
1
-
1
-
-
-
1
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
1
-
-
-
-
-
-
1
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
80992
Uotila
Expression of formaldehyde deh ...
Rattus norvegicus
Adv. Exp. Med. Biol.
414
365-371
1997
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
15
15
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
15
15
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
80991
Harms
S-Formylglutathione hydrolase ...
Paracoccus denitrificans
J. Bacteriol.
178
6296-6299
1996
-
-
-
-
-
-
-
-
-
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1
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
80993
Koivusalo
Purification and characterizat ...
Homo sapiens, Lota maculosa, Rattus norvegicus
Adv. Exp. Med. Biol.
372
427-434
1995
-
-
-
-
-
-
-
3
-
-
2
-
-
6
-
-
3
-
-
3
3
-
10
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
2
-
-
-
-
3
-
3
3
-
10
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
80989
Allais
-
Oxidation of methanol by the y ...
Komagataella pastoris, Komagataella pastoris IFP 206
Agric. Biol. Chem.
47
1509-1516
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
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-
-
-
-
-
-
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-
-
-
-
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-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
80988
Kato
-
Oxidation of formaldehyde by t ...
Geotrichum klebahnii, Kloeckera sp., Schwanniomyces vanrijiae
Agric. Biol. Chem.
46
655-661
1982
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
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-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
80987
Uotila
S-Formylglutathione hydrolase ...
Escherichia coli, Homo sapiens
Methods Enzymol.
77
320-325
1981
-
-
-
-
-
1
6
2
-
-
2
-
-
2
-
-
1
-
-
1
1
-
3
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
6
-
2
-
-
2
-
-
-
-
1
-
1
1
-
3
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
80985
Kato
Purificaton and characterizati ...
Candida boidinii, Kloeckera sp., Ogataea angusta, Ogataea trehalophila, Torulopsis pinus
Biochim. Biophys. Acta
611
323-332
1980
-
-
-
-
-
-
10
1
-
-
2
2
-
7
-
-
1
-
-
-
-
1
7
2
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
10
-
1
-
-
2
2
-
-
-
1
-
-
-
1
7
2
1
-
1
-
1
-
1
-
-
-
-
-
-
-
80986
Neben
Studies on an enzyme, S-formyl ...
Candida boidinii
Biochim. Biophys. Acta
614
81-91
1980
-
-
-
-
-
-
1
1
-
-
3
1
-
2
-
-
1
-
-
-
1
1
3
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
3
1
-
-
-
1
-
-
1
1
3
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
80983
Uotila
Purification of formaldehyde a ...
Pisum sativum
Arch. Biochem. Biophys.
196
33-45
1979
-
-
-
-
-
-
-
-
-
-
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-
1
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-
1
-
-
1
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-
1
-
-
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-
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-
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-
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-
-
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-
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-
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-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
80984
Uotila
Glutathione thiol esterases of ...
Homo sapiens
Biochim. Biophys. Acta
580
277-288
1979
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
80982
Uotila
Purification and properties of ...
Homo sapiens
J. Biol. Chem.
249
7664-7672
1974
-
-
-
-
-
1
14
2
1
-
3
-
-
2
-
-
1
-
-
2
1
1
2
1
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
14
-
2
1
-
3
-
-
-
-
1
-
2
1
1
2
1
1
-
-
-
1
-
1
-
-
-
-
-
-
-
80981
Uotila
Preparation and assay of gluta ...
Homo sapiens
Biochemistry
12
3938-3943
1973
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-
-
-
-
-
-
-
-
-
-
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-
1
-
-
1
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-
1
1
-
1
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-
1
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2
-
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-
-
-
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-
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-
-
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-
1
-
1
1
-
1
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-
-
1
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2
-
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-
-
-