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Literature summary for 3.1.2.12 extracted from
- Unique regulation of the active site of the serine esterase S-formylglutathione hydrolase (2006), J. Mol. Biol., 359, 422-432.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged wild-type and mutant enzymes in Escherichia coli and in Arabidopsis thaliana protoplasts | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C59S | site-directed mutagenesis, the mutant shows no hydrolase activity with S-formylglutathione, but with 4-methylumbelliferyl acetate in contrast to the wild-type enzyme | Arabidopsis thaliana |
| S152A | site-directed mutagenesis, completely inactive mutant | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | reversible disulfide formation at Cys59, regulatory function | Arabidopsis thaliana |  |
| iodoacetamide | - |
Arabidopsis thaliana | |
| additional information | covalent modification of Cys59 inhibits the enzyme completely | Arabidopsis thaliana | |
| NEM | - |
Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32586 | - |
x * 32586, recombinant His-tagged wild-type enzyme, mass spectrometry | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel chelate affinity chromatography | Arabidopsis thaliana |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| S-formylglutathione + H2O = glutathione + formate | Cys59 and Ser152 are involved in catalysis | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is a serine hydrolase rather than a cysteine hydrolase | Arabidopsis thaliana | ? | - |
? | |
| S-formylglutathione + H2O | - |
Arabidopsis thaliana | glutathione + formate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32586, recombinant His-tagged wild-type enzyme, mass spectrometry | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| esterase D | - |
Arabidopsis thaliana |
| serine esterase | - |
Arabidopsis thaliana |
| SFGH | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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