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show all sequences of 3.1.2.12

Molecular basis of formaldehyde detoxification: Characterization of two s-formylglutathione hydrolases from Escherichia coli, FrmB and YeiG

Gonzalez, C.F.; Proudfoot, M.; Brown, G.; Korniyenko, Y.; Mori, H.; Savchenko, A.V.; Yakunin, A.F.; J. Biol. Chem. 282, 14514-14522 (2006)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
FrmB is induced 45-75fold by formaldehyde, while YeiG is constitutively expressed
Escherichia coli
Cloned(Commentary)
Commentary
Organism
genes frmB or yaiM, and yeiG, subcloning in strain DH5alpha, overexpression in strain BL21(DE3) of His-tagged wild-type and mutant enzymes
Escherichia coli
Engineering
Amino acid exchange
Commentary
Organism
C26A
site-directed mutagenesis, the YeiG mutant shows wild-type activity
Escherichia coli
C54A
site-directed mutagenesis, the YeiG mutant shows wild-type activity
Escherichia coli
D199A
site-directed mutagenesis, the YeiG mutant shows reduced activity compared to the wild-type enzyme
Escherichia coli
D218A
site-directed mutagenesis, the YeiG mutant shows reduced activity compared to the wild-type enzyme
Escherichia coli
D223A
site-directed mutagenesis, the YeiG mutant shows no esterase activity
Escherichia coli
D255A
site-directed mutagenesis, the YeiG mutant shows 2fold reduced activity compared to the wild-type enzyme
Escherichia coli
D80A
site-directed mutagenesis, the YeiG mutant shows reduced activity compared to the wild-type enzyme
Escherichia coli
additional information
construction of FrmB and YeiG deletion mutants
Escherichia coli
S154A
site-directed mutagenesis, the YeiG mutant shows no esterase activity
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
NEM
inhibition of wild-type YeiG and YeiG mutant C26A, no inhibition of YeiG mutant C54A
Escherichia coli
PMSF
inhibition of wild-type YeiG and YeiG mutants C54A and C26A
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.29
-
4-nitrophenyl acetate
pH 8.0, 37C, recombinant FrmB
Escherichia coli
0.41
-
S-formylglutathione
pH 8.0, 37C, recombinant FrmB
Escherichia coli
0.43
-
S-formylglutathione
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
0.45
-
4-nitrophenyl acetate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
0.48
-
4-nitrophenyl propionate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
0.54
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant D255A
Escherichia coli
0.58
-
S-Lactoylglutathione
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
0.6
-
S-Lactoylglutathione
pH 8.0, 37C, recombinant FrmB
Escherichia coli
0.7
-
4-nitrophenyl butyrate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
0.8
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant D80A
Escherichia coli
0.83
-
4-nitrophenyl propionate
pH 8.0, 37C, recombinant FrmB
Escherichia coli
0.95
-
4-nitrophenyl caproate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
1.01
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant D199A
Escherichia coli
1.03
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
1.06
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant C26A
Escherichia coli
1.28
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant C54A
Escherichia coli
1.3
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant D218A
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-formylglutathione + H2O
Escherichia coli
the substrate is an intermediate in the glutathione-dependent formaldehyde detoxification pathway
glutathione + formate
-
-
?
S-formylglutathione + H2O
Escherichia coli BW25113
the substrate is an intermediate in the glutathione-dependent formaldehyde detoxification pathway
glutathione + formate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
2 enzymes FrmB and YeiG, encoded by genes frmB or yaiM, and yeiG
-
Escherichia coli BW25113
-
2 enzymes FrmB and YeiG, encoded by genes frmB or yaiM, and yeiG
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged wild-type and mutant enzymes from strain BL21(DE3) by nickel affinity chromatography
Escherichia coli
Reaction
Reaction
Commentary
Organism
S-formylglutathione + H2O = glutathione + formate
Ser145, Asp233, and His256 are essential for catalysis, representing a serine hydrolase catalytic triad, Cys26 and Cys54 are not involved in catalysis, mechanism
Escherichia coli
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
expression levels of wild-type and mutant enzymes, induced or uninduced, overview
Escherichia coli
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
substrate specificity
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-naphthyl acetate + H2O
artificial substrate of YeiG
665750
Escherichia coli
2-naphthol + acetate
-
-
-
?
2-naphthyl acetate + H2O
artificial substrate of YeiG
665750
Escherichia coli BW25113
2-naphthol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
artificial substrate of FrmB and YeiG
665750
Escherichia coli
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
artificial substrate of FrmB and YeiG
665750
Escherichia coli BW25113
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl butyrate + H2O
artificial substrate of YeiG
665750
Escherichia coli
4-nitrophenol + butyrate
-
-
-
?
4-nitrophenyl caproate + H2O
artificial substrate of YeiG
665750
Escherichia coli
4-nitrophenol + caproate
-
-
-
?
4-nitrophenyl propionate + H2O
artificial substrate of FrmB and YeiG
665750
Escherichia coli
4-nitrophenol + propionate
-
-
-
?
alpha-naphthyl acetate + H2O
-
665750
Escherichia coli
alpha-naphthol + acetate
-
-
-
-
alpha-naphthyl acetate + H2O
-
665750
Escherichia coli BW25113
alpha-naphthol + acetate
-
-
-
-
additional information
no activity by FrmB and YeiG with S-(1,2-dicarboxyethyl)glutathione, S-hexylglutathione, and S-(4-nitrobenzyl)glutathione
665750
Escherichia coli
?
-
-
-
-
additional information
no activity by FrmB and YeiG with S-(1,2-dicarboxyethyl)glutathione, S-hexylglutathione, and S-(4-nitrobenzyl)glutathione
665750
Escherichia coli BW25113
?
-
-
-
-
S-formylglutathione + H2O
the substrate is an intermediate in the glutathione-dependent formaldehyde detoxification pathway
665750
Escherichia coli
glutathione + formate
-
-
-
?
S-formylglutathione + H2O
best substrate of FrmB and YeiG
665750
Escherichia coli
glutathione + formate
-
-
-
?
S-formylglutathione + H2O
the substrate is an intermediate in the glutathione-dependent formaldehyde detoxification pathway
665750
Escherichia coli BW25113
glutathione + formate
-
-
-
?
S-lactoylglutathione + H2O
substrate of FrmB and YeiG
665750
Escherichia coli
glutathione + lactate
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
structure analysis in comparison to the Saccharomyces cerevisiae crystal structure, PDB code 1pv1, the enzyme shows a canonical alpha/beta-hydrolase fold
Escherichia coli
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Escherichia coli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.05
-
S-Lactoylglutathione
pH 8.0, 37C, recombinant FrmB
Escherichia coli
0.14
-
4-nitrophenyl propionate
pH 8.0, 37C, recombinant FrmB
Escherichia coli
0.26
-
4-nitrophenyl acetate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
0.3
-
4-nitrophenyl acetate
pH 8.0, 37C, recombinant FrmB
Escherichia coli
0.36
-
4-nitrophenyl butyrate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
0.37
-
4-nitrophenyl propionate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
0.49
-
4-nitrophenyl caproate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
1.02
-
S-Lactoylglutathione
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
2.17
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant D80A
Escherichia coli
2.23
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant D255A
Escherichia coli
2.96
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant D199A
Escherichia coli
3.13
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant D218A
Escherichia coli
3.3
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
3.81
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant D218A
Escherichia coli
5.09
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
5.1
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant C54A
Escherichia coli
5.12
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant C26A
Escherichia coli
6.51
-
S-formylglutathione
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
28.5
-
S-formylglutathione
pH 8.0, 37C, recombinant FrmB
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Escherichia coli
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
FrmB is induced 45-75fold by formaldehyde, while YeiG is constitutively expressed
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
genes frmB or yaiM, and yeiG, subcloning in strain DH5alpha, overexpression in strain BL21(DE3) of His-tagged wild-type and mutant enzymes
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C26A
site-directed mutagenesis, the YeiG mutant shows wild-type activity
Escherichia coli
C54A
site-directed mutagenesis, the YeiG mutant shows wild-type activity
Escherichia coli
D199A
site-directed mutagenesis, the YeiG mutant shows reduced activity compared to the wild-type enzyme
Escherichia coli
D218A
site-directed mutagenesis, the YeiG mutant shows reduced activity compared to the wild-type enzyme
Escherichia coli
D223A
site-directed mutagenesis, the YeiG mutant shows no esterase activity
Escherichia coli
D255A
site-directed mutagenesis, the YeiG mutant shows 2fold reduced activity compared to the wild-type enzyme
Escherichia coli
D80A
site-directed mutagenesis, the YeiG mutant shows reduced activity compared to the wild-type enzyme
Escherichia coli
additional information
construction of FrmB and YeiG deletion mutants
Escherichia coli
S154A
site-directed mutagenesis, the YeiG mutant shows no esterase activity
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
NEM
inhibition of wild-type YeiG and YeiG mutant C26A, no inhibition of YeiG mutant C54A
Escherichia coli
PMSF
inhibition of wild-type YeiG and YeiG mutants C54A and C26A
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.29
-
4-nitrophenyl acetate
pH 8.0, 37C, recombinant FrmB
Escherichia coli
0.41
-
S-formylglutathione
pH 8.0, 37C, recombinant FrmB
Escherichia coli
0.43
-
S-formylglutathione
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
0.45
-
4-nitrophenyl acetate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
0.48
-
4-nitrophenyl propionate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
0.54
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant D255A
Escherichia coli
0.58
-
S-Lactoylglutathione
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
0.6
-
S-Lactoylglutathione
pH 8.0, 37C, recombinant FrmB
Escherichia coli
0.7
-
4-nitrophenyl butyrate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
0.8
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant D80A
Escherichia coli
0.83
-
4-nitrophenyl propionate
pH 8.0, 37C, recombinant FrmB
Escherichia coli
0.95
-
4-nitrophenyl caproate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
1.01
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant D199A
Escherichia coli
1.03
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
1.06
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant C26A
Escherichia coli
1.28
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant C54A
Escherichia coli
1.3
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant D218A
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-formylglutathione + H2O
Escherichia coli
the substrate is an intermediate in the glutathione-dependent formaldehyde detoxification pathway
glutathione + formate
-
-
?
S-formylglutathione + H2O
Escherichia coli BW25113
the substrate is an intermediate in the glutathione-dependent formaldehyde detoxification pathway
glutathione + formate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged wild-type and mutant enzymes from strain BL21(DE3) by nickel affinity chromatography
Escherichia coli
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
expression levels of wild-type and mutant enzymes, induced or uninduced, overview
Escherichia coli
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
substrate specificity
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-naphthyl acetate + H2O
artificial substrate of YeiG
665750
Escherichia coli
2-naphthol + acetate
-
-
-
?
2-naphthyl acetate + H2O
artificial substrate of YeiG
665750
Escherichia coli BW25113
2-naphthol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
artificial substrate of FrmB and YeiG
665750
Escherichia coli
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
artificial substrate of FrmB and YeiG
665750
Escherichia coli BW25113
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl butyrate + H2O
artificial substrate of YeiG
665750
Escherichia coli
4-nitrophenol + butyrate
-
-
-
?
4-nitrophenyl caproate + H2O
artificial substrate of YeiG
665750
Escherichia coli
4-nitrophenol + caproate
-
-
-
?
4-nitrophenyl propionate + H2O
artificial substrate of FrmB and YeiG
665750
Escherichia coli
4-nitrophenol + propionate
-
-
-
?
alpha-naphthyl acetate + H2O
-
665750
Escherichia coli
alpha-naphthol + acetate
-
-
-
-
alpha-naphthyl acetate + H2O
-
665750
Escherichia coli BW25113
alpha-naphthol + acetate
-
-
-
-
additional information
no activity by FrmB and YeiG with S-(1,2-dicarboxyethyl)glutathione, S-hexylglutathione, and S-(4-nitrobenzyl)glutathione
665750
Escherichia coli
?
-
-
-
-
additional information
no activity by FrmB and YeiG with S-(1,2-dicarboxyethyl)glutathione, S-hexylglutathione, and S-(4-nitrobenzyl)glutathione
665750
Escherichia coli BW25113
?
-
-
-
-
S-formylglutathione + H2O
the substrate is an intermediate in the glutathione-dependent formaldehyde detoxification pathway
665750
Escherichia coli
glutathione + formate
-
-
-
?
S-formylglutathione + H2O
best substrate of FrmB and YeiG
665750
Escherichia coli
glutathione + formate
-
-
-
?
S-formylglutathione + H2O
the substrate is an intermediate in the glutathione-dependent formaldehyde detoxification pathway
665750
Escherichia coli BW25113
glutathione + formate
-
-
-
?
S-lactoylglutathione + H2O
substrate of FrmB and YeiG
665750
Escherichia coli
glutathione + lactate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
structure analysis in comparison to the Saccharomyces cerevisiae crystal structure, PDB code 1pv1, the enzyme shows a canonical alpha/beta-hydrolase fold
Escherichia coli
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Escherichia coli
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.05
-
S-Lactoylglutathione
pH 8.0, 37C, recombinant FrmB
Escherichia coli
0.14
-
4-nitrophenyl propionate
pH 8.0, 37C, recombinant FrmB
Escherichia coli
0.26
-
4-nitrophenyl acetate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
0.3
-
4-nitrophenyl acetate
pH 8.0, 37C, recombinant FrmB
Escherichia coli
0.36
-
4-nitrophenyl butyrate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
0.37
-
4-nitrophenyl propionate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
0.49
-
4-nitrophenyl caproate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
1.02
-
S-Lactoylglutathione
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
2.17
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant D80A
Escherichia coli
2.23
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant D255A
Escherichia coli
2.96
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant D199A
Escherichia coli
3.13
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant D218A
Escherichia coli
3.3
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
3.81
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant D218A
Escherichia coli
5.09
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
5.1
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant C54A
Escherichia coli
5.12
-
Alpha-naphthyl acetate
pH 8.0, 37C, recombinant YeiG mutant C26A
Escherichia coli
6.51
-
S-formylglutathione
pH 8.0, 37C, recombinant wild-type YeiG
Escherichia coli
28.5
-
S-formylglutathione
pH 8.0, 37C, recombinant FrmB
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Escherichia coli
Other publictions for EC 3.1.2.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751775
Xu
Regulation of S-formylglutath ...
Homo sapiens, Mus musculus
Oncotarget
8
88259-88275
2017
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2
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2
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4
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2
2
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2
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2
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4
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2
2
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2
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2
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751579
Li
Esterase D enhances type I in ...
Sus scrofa
Mol. Immunol.
75
112-121
2016
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-
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3
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80981
Uotila
Preparation and assay of gluta ...
Homo sapiens
Biochemistry
12
3938-3943
1973
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