BRENDA - Enzyme Database show
show all sequences of 3.1.2.12

Cloning and characterization of an S-formylglutathione hydrolase from Arabidopsis thaliana

Kordic, S.; Cummins, I.; Edwards, R.; Arch. Biochem. Biophys. 399, 232-238 (2002)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
not affected by Ca2+, Mn2+, Mg2+, and EDTA, not affected by methanol, phenobarbital and dichlormid
Arabidopsis thaliana
Cloned(Commentary)
Commentary
Organism
DNA and amino acid sequence determinationand analysis, expression in Escherichia coli as C-terminally His-tagged enzyme
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
90% reduced activity at 0.01 mM
Arabidopsis thaliana
additional information
no inhibition by PMSF, not affected by Ca2+, Mn2+, Mg2+, and EDTA, not affected by methanol, phenobarbital and dichlormid
Arabidopsis thaliana
N-ethylmaleimide
complete inhibition at 1 mM
Arabidopsis thaliana
Zn2+
50% reduced activity at 0.01 mM
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
fluorescein diacetate
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
0.12
-
4-yethylumbelliferyl acetate
recombinant enzyme, pH 7.5, 37°C
Arabidopsis thaliana
0.13
-
S-formylglutathione
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
0.15
-
S-acetylglutathione
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
0.54
-
beta-naphthyl acetate
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
0.57
-
Alpha-naphthyl acetate
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
1.02
-
4-nitrophenyl acetate
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31700
-
2 * 31700, recombinant enzyme, amino acid sequence calculation without His-tag
Arabidopsis thaliana
66000
-
recombinant enzyme, gel filtration
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-formylglutathione + H2O
Arabidopsis thaliana
enzyme is involved in formaldehyde detoxification
glutathione + formate
-
Arabidopsis thaliana
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli
Arabidopsis thaliana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Arabidopsis thaliana
-
plant
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.08
-
recombinant enzyme, substrate fluorescein diacetate
Arabidopsis thaliana
1.3
-
recombinant enzyme, substrate beta-naphthyl acetate
Arabidopsis thaliana
5.7
-
recombinant enzyme, substrate 4-nitrophenyl acetate
Arabidopsis thaliana
10.4
-
recombinant enzyme, substrate S-formylglutathione
Arabidopsis thaliana
14.5
-
recombinant enzyme, substrate S-acetylglutathione
Arabidopsis thaliana
15.2
-
recombinant enzyme, substrate alpha-naphthyl acetate
Arabidopsis thaliana
36.3
-
recombinant enzyme, substrate 4-methylumbelliferyl acetate
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-methylumbelliferyl acetate + H2O
-
649455
Arabidopsis thaliana
4-methylumbelliferol + acetate
-
649455
Arabidopsis thaliana
?
4-nitrophenyl acetate + H2O
-
649455
Arabidopsis thaliana
4-nitrophenol + acetate
-
649455
Arabidopsis thaliana
?
alpha-naphthyl acetate + H2O
-
649455
Arabidopsis thaliana
alpha-naphthol + acetate
-
649455
Arabidopsis thaliana
?
beta-naphthyl acetate + H2O
-
649455
Arabidopsis thaliana
beta-naphthol + acetate
-
649455
Arabidopsis thaliana
?
fluorescein diacetate + H2O
-
649455
Arabidopsis thaliana
?
-
649455
Arabidopsis thaliana
?
S-acetylglutathione + H2O
-
649455
Arabidopsis thaliana
glutathione + acetate
-
649455
Arabidopsis thaliana
?
S-formylglutathione + H2O
-
649455
Arabidopsis thaliana
glutathione + formate
-
649455
Arabidopsis thaliana
?
S-formylglutathione + H2O
enzyme is involved in formaldehyde detoxification
649455
Arabidopsis thaliana
glutathione + formate
-
649455
Arabidopsis thaliana
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 31700, recombinant enzyme, amino acid sequence calculation without His-tag
Arabidopsis thaliana
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Arabidopsis thaliana
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
fully stable up to, rapid inactivation at higher temperatures
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at, except with substrate 4-methylumbelliferyl acetate
Arabidopsis thaliana
7.6
8
optimum
Arabidopsis thaliana
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Arabidopsis thaliana
isoelectric focusing
-
6
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
not affected by Ca2+, Mn2+, Mg2+, and EDTA, not affected by methanol, phenobarbital and dichlormid
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determinationand analysis, expression in Escherichia coli as C-terminally His-tagged enzyme
Arabidopsis thaliana
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
90% reduced activity at 0.01 mM
Arabidopsis thaliana
additional information
no inhibition by PMSF, not affected by Ca2+, Mn2+, Mg2+, and EDTA, not affected by methanol, phenobarbital and dichlormid
Arabidopsis thaliana
N-ethylmaleimide
complete inhibition at 1 mM
Arabidopsis thaliana
Zn2+
50% reduced activity at 0.01 mM
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
fluorescein diacetate
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
0.12
-
4-yethylumbelliferyl acetate
recombinant enzyme, pH 7.5, 37°C
Arabidopsis thaliana
0.13
-
S-formylglutathione
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
0.15
-
S-acetylglutathione
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
0.54
-
beta-naphthyl acetate
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
0.57
-
Alpha-naphthyl acetate
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
1.02
-
4-nitrophenyl acetate
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31700
-
2 * 31700, recombinant enzyme, amino acid sequence calculation without His-tag
Arabidopsis thaliana
66000
-
recombinant enzyme, gel filtration
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-formylglutathione + H2O
Arabidopsis thaliana
enzyme is involved in formaldehyde detoxification
glutathione + formate
-
Arabidopsis thaliana
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Arabidopsis thaliana
-
plant
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.08
-
recombinant enzyme, substrate fluorescein diacetate
Arabidopsis thaliana
1.3
-
recombinant enzyme, substrate beta-naphthyl acetate
Arabidopsis thaliana
5.7
-
recombinant enzyme, substrate 4-nitrophenyl acetate
Arabidopsis thaliana
10.4
-
recombinant enzyme, substrate S-formylglutathione
Arabidopsis thaliana
14.5
-
recombinant enzyme, substrate S-acetylglutathione
Arabidopsis thaliana
15.2
-
recombinant enzyme, substrate alpha-naphthyl acetate
Arabidopsis thaliana
36.3
-
recombinant enzyme, substrate 4-methylumbelliferyl acetate
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-methylumbelliferyl acetate + H2O
-
649455
Arabidopsis thaliana
4-methylumbelliferol + acetate
-
649455
Arabidopsis thaliana
?
4-nitrophenyl acetate + H2O
-
649455
Arabidopsis thaliana
4-nitrophenol + acetate
-
649455
Arabidopsis thaliana
?
alpha-naphthyl acetate + H2O
-
649455
Arabidopsis thaliana
alpha-naphthol + acetate
-
649455
Arabidopsis thaliana
?
beta-naphthyl acetate + H2O
-
649455
Arabidopsis thaliana
beta-naphthol + acetate
-
649455
Arabidopsis thaliana
?
fluorescein diacetate + H2O
-
649455
Arabidopsis thaliana
?
-
649455
Arabidopsis thaliana
?
S-acetylglutathione + H2O
-
649455
Arabidopsis thaliana
glutathione + acetate
-
649455
Arabidopsis thaliana
?
S-formylglutathione + H2O
-
649455
Arabidopsis thaliana
glutathione + formate
-
649455
Arabidopsis thaliana
?
S-formylglutathione + H2O
enzyme is involved in formaldehyde detoxification
649455
Arabidopsis thaliana
glutathione + formate
-
649455
Arabidopsis thaliana
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 31700, recombinant enzyme, amino acid sequence calculation without His-tag
Arabidopsis thaliana
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Arabidopsis thaliana
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
fully stable up to, rapid inactivation at higher temperatures
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at, except with substrate 4-methylumbelliferyl acetate
Arabidopsis thaliana
7.6
8
optimum
Arabidopsis thaliana
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Arabidopsis thaliana
isoelectric focusing
-
6
Other publictions for EC 3.1.2.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Xu
Regulation of S-formylglutath ...
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2
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2
2
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2
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2
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4
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2
2
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2
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2
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751579
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112-121
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3
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1
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1
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-
751889
Huetten
Activity profiling reveals ch ...
Arabidopsis thaliana
Plant Physiol. Biochem.
97
36-43
2015
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7
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1
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1
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1
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1
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1
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-
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-
-
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-
-
1
-
-
1
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731220
Legler
A role for His-160 in peroxide ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
528
7-20
2012
-
-
-
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9
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2
13
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-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
1
-
-
15
1
-
-
-
3
-
-
-
-
-
-
-
9
-
-
2
3
13
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
15
1
-
-
-
-
-
-
-
15
15
715774
Parracino
Towards nanoscale biomedical d ...
Pseudoalteromonas haloplanktis, Pseudoalteromonas haloplanktis TAC 125
J. Fluoresc.
21
663-672
2011
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11
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1
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2
1
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1
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1
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1
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1
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1
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-
2
1
-
-
-
-
1
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-
1
-
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-
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-
707759
Alterio
Crystal structure of an S-form ...
Pseudoalteromonas haloplanktis, Pseudoalteromonas haloplanktis TAC 125
Biopolymers
93
669-677
2010
-
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1
1
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-
-
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2
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11
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1
-
-
-
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-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
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-
2
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-
-
-
1
-
-
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-
8
1
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-
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-
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-
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716804
Liu
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Pseudomonas putida
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1
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691781
Tuin
Activity-based protein profili ...
Homo sapiens
Chem. Res. Toxicol.
22
683-689
2009
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2
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1
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1
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3
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2
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2
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1
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3
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2
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694178
Kaschani
Diversity of serine hydrolase ...
Arabidopsis thaliana
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8
1082-1093
2009
1
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6
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1
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1
1
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1
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1
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1
1
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-
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-
710495
van Straaten
The structure of a putative S- ...
Agrobacterium tumefaciens
Protein Sci.
18
2196-2202
2009
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1
1
2
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1
6
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1
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7
1
-
-
-
6
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1
-
1
2
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1
-
6
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1
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-
7
1
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6
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-
6
6
691005
Legler
Structural characterization an ...
Saccharomyces cerevisiae
Biochemistry
47
9592-9601
2008
-
-
1
1
11
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3
24
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-
3
-
-
3
1
-
1
-
-
-
-
-
5
2
-
-
-
24
-
-
-
-
-
-
-
-
-
1
-
1
11
-
-
3
-
24
-
-
3
-
-
1
-
1
-
-
-
-
5
2
-
-
-
24
-
-
-
-
-
-
-
-
-
-
682300
Cummins
Structure activity studies wit ...
Arabidopsis thaliana
Phytochemistry
68
811-818
2007
-
-
1
-
-
-
-
14
-
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6
-
-
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-
-
-
-
-
14
-
-
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-
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-
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1
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-
-
-
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-
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14
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-
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14
-
-
-
-
-
-
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682315
Cummins
Identification of a carboxyles ...
Arabidopsis thaliana
Plant Biotechnol. J.
5
354-359
2007
-
1
-
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1
-
1
-
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4
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-
-
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1
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1
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1
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1
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1
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-
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-
665750
Gonzalez
Molecular basis of formaldehyd ...
Escherichia coli, Escherichia coli BW25113
J. Biol. Chem.
282
14514-14522
2006
1
-
1
-
9
-
2
17
-
-
-
2
-
31
-
-
1
1
-
1
1
-
15
1
1
-
-
19
1
-
-
-
-
-
-
1
-
1
-
-
9
-
-
2
-
17
-
-
-
2
-
-
-
1
-
1
1
-
15
1
1
-
-
19
1
-
-
-
-
-
-
-
-
-
666081
Cummins
Unique regulation of the activ ...
Arabidopsis thaliana
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Kato
Purificaton and characterizati ...
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Neben
Studies on an enzyme, S-formyl ...
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Uotila
Purification of formaldehyde a ...
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Uotila
Glutathione thiol esterases of ...
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Uotila
Purification and properties of ...
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Uotila
Preparation and assay of gluta ...
Homo sapiens
Biochemistry
12
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