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Literature summary for 3.1.2.1 extracted from
- Acetate generation in rat liver mitochondria: acetyl-CoA hydrolase activity is demonstrated by 3-ketoacyl-CoA thiolase (2006), Biochim. Biophys. Acta, 1761, 17-23.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is induced in mitochondria by di(2-ethylhexyl)phthalate | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | exclusively | Rattus norvegicus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + H2O | Rattus norvegicus | - |
CoA + acetate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male Spargue-Dawley rats | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 27.2fold from liver mitochondria in a 5-step chromatographical procedure | Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetyl-CoA + H2O = CoA + acetate | the bifunctional enzyme is identical with a 3-ketoacyl-CoA thiolase isozyme, i.e. THL, EC 2.3.1.9, determined by internal peptide amino acid sequence comparison | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brown adipose tissue | - |
Rattus norvegicus | - |
| heart | - |
Rattus norvegicus | - |
| intestine | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + H2O | - |
Rattus norvegicus | CoA + acetate | - |
? | |
| acetyl-CoA + H2O | highly preferred substrate | Rattus norvegicus | CoA + acetate | - |
? | |
| additional information | substrate and chain-length specificity of the mitochondrial enzyme, overview | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACH | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
assay at | Rattus norvegicus |
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Release 2023.1 (January 2023)
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