Cloned (Comment) | Organism |
---|---|
gene nurA, DNA and amino acid sequence determination and analysis, the nurA gene is arranged in an operon-like structure with mre11, rad50, and herA genes, expression with StoSSB in the two-hybrid system in yeast AH109 | Sulfurisphaera tokodaii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
single-stranded DNA-binding protein | i.e. StoSSB, from the hyperthermophilic archaeon Sulfolobus tokodaii, StoSSB inhibits the exonuclease activity of StoNurA on ssDNA and dsDNA | Sulfurisphaera tokodaii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Sulfurisphaera tokodaii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Sulfurisphaera tokodaii | NurA may function closely together with SSB in DNA transactions in archaea | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | - |
gene nurA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | NurA may function closely together with SSB in DNA transactions in archaea | Sulfurisphaera tokodaii | ? | - |
? | |
additional information | NurA has ssDNA endonuclease, 5'-3' ssDNA exonuclease, and 5'-3' dsDNA exonuclease activities, overview. NurA is closely linked to Mre11 and Rad50 homologues, it interacts with single-stranded DNA-binding protein, StoSSB, from the hyperthermophilic archaeon Sulfolobus tokodaii, determination by pull-down assay using Ni-NTA agarose beads and MALDI-TOF mass spectrometry, and yeast two-hybrid and co-immunoprecipitation analysis, overview. Substrates are 34-bp dsDNA and M13mp18 ssDNA | Sulfurisphaera tokodaii | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Sulfurisphaera tokodaii |