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Literature summary for 3.1.16.1 extracted from

  • Mitkova, A.V.; Bakalova, A.T.; Stoynov, S.S.; Dolapchiev, L.B.
    Oligomeric protein structure of beef spleen exonuclease (1998), Arch. Biochem. Biophys., 351, 236-242.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
alpha2beta2, 2 * 25000 + 2 * 55000, intermolecular disulfide bonds, no activity of subunits alpha or beta, but 3.1 times higher specific activity of tetramer in comparison with alpha,beta dimer, SDS-polyacrylamide gel electrophoresis in presence of mercaptoethanol Bos taurus
55000
-
alpha2beta2, 2 * 25000 + 2 * 55000, intermolecular disulfide bonds, no activity of subunits alpha or beta, but 3.1 times higher specific activity of tetramer in comparison with alpha,beta dimer, SDS-polyacrylamide gel electrophoresis in presence of mercaptoethanol Bos taurus
160000
-
SDS-polyacrylamide gel electrophoresis Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polynucleotides + H2O Bos taurus involvement in DNA repair and replication, genetic recombination, elimination of damaged nucleotides 3'-phosphomononucleotides
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein both monomers glycosylated Bos taurus

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polynucleotides + H2O involvement in DNA repair and replication, genetic recombination, elimination of damaged nucleotides Bos taurus 3'-phosphomononucleotides
-
?
thymidine 3'-(4-nitrophenyl)phosphate + H2O
-
Bos taurus thymidine 3'-phosphate + 4-nitrophenol
-
?

Subunits

Subunits Comment Organism
tetramer alpha2beta2, 2 * 25000 + 2 * 55000, intermolecular disulfide bonds, no activity of subunits alpha or beta, but 3.1 times higher specific activity of tetramer in comparison with alpha,beta dimer, SDS-polyacrylamide gel electrophoresis in presence of mercaptoethanol Bos taurus

Synonyms

Synonyms Comment Organism
phosphodiesterase II
-
Bos taurus