Cloned (Comment) | Organism |
---|---|
gene PF2046 | Pyrococcus furiosus |
Crystallization (Comment) | Organism |
---|---|
enzyme PF2046 complexed with an oligonucleotide of four thymidine nucleotides (dT4), X-ray diffraction structure determination and analysis | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pyrococcus furiosus | RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate | ? | - |
? | |
additional information | Pyrococcus furiosus ATCC 43587 | RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate | ? | - |
? | |
additional information | Pyrococcus furiosus Vc1 | RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate | ? | - |
? | |
additional information | Pyrococcus furiosus JCM 8422 | RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8TZE9 | - |
- |
Pyrococcus furiosus ATCC 43587 | Q8TZE9 | - |
- |
Pyrococcus furiosus JCM 8422 | Q8TZE9 | - |
- |
Pyrococcus furiosus Vc1 | Q8TZE9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate | Pyrococcus furiosus | ? | - |
? | |
additional information | RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate | Pyrococcus furiosus ATCC 43587 | ? | - |
? | |
additional information | RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate | Pyrococcus furiosus Vc1 | ? | - |
? | |
additional information | RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate | Pyrococcus furiosus JCM 8422 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PF2046 | - |
Pyrococcus furiosus |
RNase H | - |
Pyrococcus furiosus |
General Information | Comment | Organism |
---|---|---|
additional information | in the structure of PF2046 complexed with an oligonucleotide of four thymidine nucleotides (dT4), PF2046 accommodates dT4 tightly in a groove and imposes steric hindrance on dT4 mainly by residue Phe220 such that dT4 assumes the A-form. As poly-dA prefer B-form due to the stereochemical restrictions, the A-form ssDNA binding by PF2046 disfavors the processing of poly-dA. Phe220 variants display reduced activity toward poly-dA and the A-form appears to be a prerequisite for the processing by PF2046 | Pyrococcus furiosus |