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Literature summary for 3.1.13.3 extracted from

  • Orr, M.W.; Donaldson, G.P.; Severin, G.B.; Wang, J.; Sintim, H.O.; Waters, C.M.; Lee, V.T.
    Oligoribonuclease is the primary degradative enzyme for pGpG in Pseudomonas aeruginosa that is required for cyclic-di-GMP turnover (2015), Proc. Natl. Acad. Sci. USA, 112, E5048-E5057 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.13.3

Cloned(Commentary)

Cloned (Comment) Organism
gene orn, quantitative RT-PCR expression analysis Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
additional information generation of enzyme mutant DELTAorn. The lysates from DELTAorn show 25fold decrease in 5'-phosphoguanylyl-(3',5')-guanosine (pGpG) hydrolysis. Complementation with wild-type, but not active site mutants, restores hydrolysis. Accumulation of pGpG in the DELTAorn strain inhibits PDE-As, increasing cyclic-diguanylate (c-di-GMP) concentration. Increased transcription from the cyclic-diguanylate-regulated pel promoter is observed. Additionally, the cyclic-diguanylate-governed auto-aggregation and biofilm phenotypes are elevated in the DELTAorn strain in a pel-dependent manner. Detection of elevated levels of pGpG and cyclic-diguanylate in the DELTAorn strain Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-phosphoguanylyl-(3',5')-guanosine + H2O Pseudomonas aeruginosa i.e. pGpG GMP + guanosine
-
 ?
additional information Pseudomonas aeruginosa oligoribonuclease (Orn) is an exoribonuclease that hydrolyzes two- to five-nucleotide-long RNAs. Orn degrades short oligoRNAs regardless of sequence ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q02F67
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-phosphoguanylyl-(3',5')-guanosine + H2O i.e. pGpG Pseudomonas aeruginosa GMP + guanosine
-
 ?
additional information oligoribonuclease (Orn) is an exoribonuclease that hydrolyzes two- to five-nucleotide-long RNAs. Orn degrades short oligoRNAs regardless of sequence Pseudomonas aeruginosa ?
-
 ?

Synonyms

Synonyms Comment Organism
oligoribonuclease
-
 Pseudomonas aeruginosa
ORN
-
 Pseudomonas aeruginosa

General Information

General Information Comment Organism
malfunction the lysates from DELTAorn show 25fold decrease in 5'-phosphoguanylyl-(3',5')-guanosine (pGpG) hydrolysis. Complementation with wild-type, but not active site mutants, restores hydrolysis. Accumulation of pGpG in the DELTAorn strain inhibits PDE-As, increasing cyclic-diguanylate (c-di-GMP) concentration. Increased transcription from the cyclic-diguanylate-regulated pel promoter is observed. Additionally, the cyclic-diguanylate-governed auto-aggregation and biofilm phenotypes are elevated in the DELTAorn strain in a pel-dependent manner. Detection of elevated levels of pGpG and cyclic-diguanylate in the DELTAorn strain. Phenotype, overview Pseudomonas aeruginosa
physiological function oligoribonuclease (Orn), an exoribonuclease that hydrolyzes two- to five-nucleotide-long RNAs, is the primary enzyme responsible for degrading 5'-phosphoguanylyl-(3',5')-guanosine (pGpG) in cells. Orn binds to pGpG specifically and can cleave it into GMP. pGpG inhibits RocR phosphodiesterase activity by binding to the active site and competing for cyclic diguanylate binding in the active site, and excess pGpG extends cyclic diguanylate half-life in vitro Pseudomonas aeruginosa