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Literature summary for 3.1.13.3 extracted from

  • Chen, F.; Chen, G.; Liu, Y.; Jin, Y.; Cheng, Z.; Liu, Y.; Yang, L.; Jin, S.; Wu, W.
    Pseudomonas aeruginosa oligoribonuclease contributes to Tolerance to ciprofloxacin by regulating pyocin biosynthesis (2017), Antimicrob. Agents Chemother., 61, e02256-16 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.13.3

Cloned(Commentary)

Cloned (Comment) Organism
gene orn, quantitative RT-PCR expression analysis Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
additional information generation of enzyme mutant DELTAorn, mRNA levels of pyocin biosynthesis genes in the DELTAorn mutant compared to those in wild-type strain PA14. Mutation of orn drastically increases bacterial susceptibility to quinolones but not to tetracycline, aminoglycoside, or beta-lactam antibiotics. Upregulation of pyocin genes contributes to increased susceptibility to ciprofloxacin in the orn mutant. PrtR stability is reduced by the mutation of orn Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-phosphoguanylyl-(3',5')-guanosine + H2O Pseudomonas aeruginosa i.e. pGpG GMP + guanosine
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 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q02F67
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-phosphoguanylyl-(3',5')-guanosine + H2O i.e. pGpG Pseudomonas aeruginosa GMP + guanosine
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 ?

Synonyms

Synonyms Comment Organism
oligoribonuclease
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 Pseudomonas aeruginosa
ORN
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 Pseudomonas aeruginosa

General Information

General Information Comment Organism
evolution Orn is a highly conserved 3'-to-5' exonuclease Pseudomonas aeruginosa
malfunction mutation of gene orn leads to the accumulation of pGpG, which inhibits the function of glutamate-alanine-leucine (EAL) domain-containing enzymes, resulting in increased levels of cyclic-di-GMP and the consequent hyperbiofilm phenotype. EAL domain-containing enzymes hydrolyze cyclic-di-GMP to 5'-phosphoguanylyl-(3',5')-guanosine (pGpG). Mutation of orn drastically increases bacterial susceptibility to quinolones but not to tetracycline, aminoglycoside, or beta-lactam antibiotics. Upregulation of pyocin genes contributes to increased susceptibility to ciprofloxacin in the orn mutant. PrtR stability is reduced by the mutation of orn. Expression levels of prtN and PA0613 in wild-type and DELTAorn mutant strains PA14, phenotypes, overview Pseudomonas aeruginosa
physiological function bacterial oligoribonuclease (Orn) is a conserved 3'-to-5' exonuclease. In Pseudomonas aeruginosa, Orn plays a major role in the hydrolysis of pGpG, which is required for cyclic-di-GMP homeostasis. Orn is involved in the degradation of nanoRNAs, which can alter global gene expression by serving as transcription initiation primers. And Orn is required for the type III secretion system and pathogenesis of Pseudomonas aeruginosa, indicating a role of Orn in the bacterial response to environmental stimuli. Orn is required for the tolerance of Pseudomonas aeruginosa to ciprofloxacin, role of Orn in bacterial resistance to antibiotics. And role of Orn in genome integrity and bacterial resistance to quinolones. Oligoribonuclease is required for bacterial resistance to fluoroquinolones Pseudomonas aeruginosa