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Literature summary for 3.1.13.3 extracted from

  • Liu, M.F.; Cescau, S.; Mechold, U.; Wang, J.; Cohen, D.; Danchin, A.; Boulouis, H.J.; Biville, F.
    Identification of a novel nanoRNase in Bartonella (2012), Microbiology, 158, 886-895.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bartonella birtlesii

Inhibitors

Inhibitors Comment Organism Structure
3'-phosphoadenosine 5'-phosphate in vitro inhibitory at 0.05 mM Bartonella birtlesii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ assay in presence of 5 mM Mg2+ or Mn2+. Activity is similar in the presence of manganese or magnesium Bartonella birtlesii
Mn2+ assay in presence of 5 mM Mg2+ or Mn2+. Activity is similar in the presence of manganese or magnesium Bartonella birtlesii
additional information Ca2+ cannot substitute for Mn2+ or Mg2+ Bartonella birtlesii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
x * 24000, SDS-PAGE of recombinant protein Bartonella birtlesii

Organism

Organism UniProt Comment Textmining
Bartonella birtlesii G4VUY7
-
-
Bartonella birtlesii IBS 325 G4VUY7
-
-
Bartonella henselae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
24mer RNA + H2O
-
Bartonella birtlesii nucleoside 5'-phosphates
-
?
24mer RNA + H2O
-
Bartonella birtlesii IBS 325 nucleoside 5'-phosphates
-
?
5'Cy5-AAA3' + H2O better substrate than 5'Cy5-CCCCC3' Bartonella birtlesii ?
-
?
5'Cy5-AAA3' + H2O better substrate than 5'Cy5-CCCCC3' Bartonella birtlesii IBS 325 ?
-
?
5'Cy5-AAAAA3' + H2O better substrate than 5'Cy5-CCCCC3' Bartonella birtlesii ?
-
?
5'Cy5-AAAAA3' + H2O better substrate than 5'Cy5-CCCCC3' Bartonella birtlesii IBS 325 ?
-
?
5'Cy5-CCC3' + H2O 5'Cy5-AAA3' is a better substrate than 5'Cy5-CCC3' Bartonella birtlesii ?
-
?
5'Cy5-CCC3' + H2O 5'Cy5-AAA3' is a better substrate than 5'Cy5-CCC3' Bartonella birtlesii IBS 325 ?
-
?
5'Cy5-CCCCC3' + H2O 5'Cy5-AAAAA3' is a better substrate than 5'Cy5-CCCCC3' Bartonella birtlesii ?
-
?
additional information oligo(A) RNA substrates are preferred over oligo(C) substrates, and RA 5mers are preferred over RNA 3mers Bartonella birtlesii ?
-
?
additional information oligo(A) RNA substrates are preferred over oligo(C) substrates, and RA 5mers are preferred over RNA 3mers Bartonella birtlesii IBS 325 ?
-
?

Subunits

Subunits Comment Organism
? x * 24000, SDS-PAGE of recombinant protein Bartonella birtlesii

Synonyms

Synonyms Comment Organism
nanoRNase
-
Bartonella henselae
nanoRNase
-
Bartonella birtlesii
NrnC
-
Bartonella henselae
NrnC
-
Bartonella birtlesii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Bartonella birtlesii

General Information

General Information Comment Organism
physiological function expression of isoform NrnC in Escherichia coli can complement lack of oligoribonuclease Orn. Knock down of NrnC decreases the growth ability of Bartonella henselae Bartonella henselae