Cloned (Comment) | Organism |
---|---|
wild-type ornA and the ornA (D130A) point mutant are placed under the control of the strong, constitutive ermE promoter in the hygromycin-resistant, attP-integrative vector pIJ10275. The resultant plasmids (pJS93 and pJS94) introduced into Streptomyces coelicolor J3411 (DELTAornA::apr) by conjugation with Escherichia coli strain ET12567 (pUZ8002) to yield Streptomyces coelicolor J3411 (pJS93) and Streptomyces coelicolor J3411 (pJS94) | Streptomyces coelicolor |
Protein Variants | Comment | Organism |
---|---|---|
D130A | pJS94, encoding the catalytically inactive point mutant OrnA D130A does not suppress the phenotype of the Streptomyces coelicolor J3411 (DELTAornA::apr) null mutant | Streptomyces coelicolor |
additional information | oligoribonuclease null mutant J3411 (DELTAornA::apr), reveals a conditional defect in morphological differentiation that is reminiscent of bldA. PpJS93, containing ornA under the control of a constitutive promoter, completely suppresses the phenotype of Streptomyces coelicolor J3411 (DELTAornA::apr), but pJS94 (encoding catalytically inactive OrnA D130A) does not | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Streptomyces coelicolor | degradation of RNA oligomers by oligoribonuclease is critical for completion of the Streptomyces coelicolor life cycle, but expression of ornA is not likely to be contigent on bldA-dependent translation of adpA | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | degradation of RNA oligomers by oligoribonuclease is critical for completion of the Streptomyces coelicolor life cycle, but expression of ornA is not likely to be contigent on bldA-dependent translation of adpA | Streptomyces coelicolor | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
oligoribonuclease | - |
Streptomyces coelicolor |