Cloned (Comment) | Organism |
---|---|
into pMCSG7 vector | Xanthomonas campestris |
Crystallization (Comment) | Organism |
---|---|
by sitting-drop vapor diffusion method, to a resolution of 2.1 A, orn monomer comprises 9 alpha-helices and 5 beta-strands, helix H is oriented opposingly from similar helices in all reported 3'-5' DNases discovered so far, possibly to prevent steric hindrance of accommodating oligoribonucleotide substrates. Amino acids Leu135, Leu143, Ile140, and Leu174 form a hydrophobic cluster, side chains of Glu142/Arg133 and Thr139/Asp136 form salt bridges and H-bonds in the dimer interface, while side chains of Arg145, and Lys157 interact with the ribose 2'-OH or phosphate oxygen atoms of U5 substrates | Xanthomonas campestris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | one magnesium ion, coordinating well with the DEDDh active site carboxylate groups of Asp15, Glu17, Asp166, and the oxygen atom of U51Op group | Xanthomonas campestris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas campestris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
more than 99% pure | Xanthomonas campestris |
Subunits | Comment | Organism |
---|---|---|
homodimer | crystallography | Xanthomonas campestris |
Synonyms | Comment | Organism |
---|---|---|
XC847 | - |
Xanthomonas campestris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
active site highly acidic and substrate binding site highly basic | Xanthomonas campestris |