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Literature summary for 3.1.13.3 extracted from

  • Mechold, U.; Fang, G.; Ngo, S.; Ogryzko, V.; Danchin, A.
    YtqI from Bacillus subtilis has both oligoribonuclease and pAp-phosphatase activity (2007), Nucleic Acids Res., 35, 4552-4561.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
additional information YtqI, in addition to oligoribonuclease activity, has 3'-phosphoadenosine 5'-phosphate-phosphatase activity in vitro. YtqI is able to complement both orn and cysQ mutants in Escherichia coli Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
expression of his-tagged YtqI under control of the arabinose-inducible promoter Para Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information ORN mutant, shows growth defect, YtqI from Bacillus subtilis is able to complement the mutant Escherichia coli
additional information ytqI mutant, shows impairment of growth in the absence of cysteine Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
3'-phosphoadenosine 5'-phosphate inhibits YtqI-catalyzed degradation of nanoRNA. At 0.1, 0.2 or 0.5 mM, the activity of YtqI based on the conversion of 5-mer into monomers in 30 min dropps to 28, 4 and 1%, respectively Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenosine 5'-phosphate + H2O
-
Bacillus subtilis AMP + phosphate
-
?
RNA + H2O degrades RNA oligonucleotides with preference for 3-mers Bacillus subtilis nucleoside 5'-phosphate + ?
-
?

Synonyms

Synonyms Comment Organism
ORN
-
Escherichia coli
YtqI
-
Bacillus subtilis