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Literature summary for 3.1.13.3 extracted from

  • Datta, A.K.; Niyogi, S.N.
    A novel oligoribonuclease of Escherichia coli. II. Mechanism of action (1975), J. Biol. Chem., 250, 7313-7319.
    View publication on PubMed
Search for more literature for 3.1.13.3

Inhibitors

Inhibitors Comment Organism Structure
5'-AMP
-
 Escherichia coli
5'-GMP
-
 Escherichia coli
ADP
-
 Escherichia coli
ATP
-
 Escherichia coli
GDP
-
 Escherichia coli
GTP
-
 Escherichia coli
UTP slight Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 (Ap)6
-
 Escherichia coli
0.021
-
 (Ap)4
-
 Escherichia coli
0.05
-
 (Ap)3
-
 Escherichia coli
0.25
-
 (Ap)2
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oligonucleotides + H2O Escherichia coli
-
 nucleoside 5'-phosphates
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Ap)2 + H2O
-
 Escherichia coli ?
-
 ?
(Ap)3 + H2O
-
 Escherichia coli ?
-
 ?
(Ap)4 + H2O
-
 Escherichia coli ?
-
 ?
(Ap)6 + H2O
-
 Escherichia coli ?
-
 ?
CpC + H2O
-
 Escherichia coli 5'-CMP + cytidine
-
 ?
CpU + H2O
-
 Escherichia coli 3'-CMP + uridine
-
 ?
oligonucleotides + H2O
-
 Escherichia coli nucleoside 5'-phosphates
-
 ?
oligonucleotides + H2O processive degradation Escherichia coli nucleoside 5'-phosphates
-
 ?
oligonucleotides + H2O however higher affinity to longer chains Escherichia coli nucleoside 5'-phosphates
-
 ?
oligonucleotides + H2O exonuclease: 3`to 5'-direction Escherichia coli nucleoside 5'-phosphates
-
 ?
oligonucleotides + H2O reaction rate inversely proportional to chain length of substrate Escherichia coli nucleoside 5'-phosphates
-
 ?
oligonucleotides + H2O initial site of attack: 3'-hydroxyl end Escherichia coli nucleoside 5'-phosphates
-
 ?
oligonucleotides + H2O also hydrolyses NAD+ to NMN and AMP, mechanism Escherichia coli nucleoside 5'-phosphates
-
 ?
UpC + H2O
-
 Escherichia coli 5'-CMP + 5'-UMP
-
 ?
UpU + H2O
-
 Escherichia coli 5'-UMP
-
 ?