Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3'-AMP | slight | Rattus norvegicus | |
5'-AMP | - |
Rattus norvegicus | |
5'-UMP | - |
Rattus norvegicus | |
ADP | - |
Rattus norvegicus | |
ATP | - |
Rattus norvegicus | |
beta-mercaptoethanol | slight | Rattus norvegicus | |
CuCl2 | slight | Rattus norvegicus | |
EDTA | - |
Rattus norvegicus | |
HgCl2 | - |
Rattus norvegicus | |
p-chloromercuribenzoate | slight | Rattus norvegicus | |
UDP | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
p-nitrophenyl uridine 5'-monophosphate | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation | Rattus norvegicus | |
Co2+ | activation | Rattus norvegicus | |
K+ | activates at high concentration | Rattus norvegicus | |
Mg2+ | activation | Rattus norvegicus | |
Na+ | activates at high concentration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oligonucleotides + H2O | Rattus norvegicus | - |
nucleoside 5'-phosphates | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
p-nitrophenyl uridine 5'-monophosphate | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | hydrolyzed very slowly, not: ADP, UDP, ATP, UTP | Rattus norvegicus | nucleoside 5'-phosphates | - |
? | |
NAD+ + H2O | - |
Rattus norvegicus | NMN + AMP | - |
? | |
oligonucleotides + H2O | - |
Rattus norvegicus | nucleoside 5'-phosphates | - |
? | |
oligonucleotides + H2O | no base specificity | Rattus norvegicus | nucleoside 5'-phosphates | - |
? | |
oligonucleotides + H2O | initial site of attack: 3'-hydroxyl end | Rattus norvegicus | nucleoside 5'-phosphates | - |
? | |
p-nitrophenyl uridine 5'-monophosphate + H2O | - |
Rattus norvegicus | p-nitrophenol + 5'-UMP | - |
? | |
p-nitrophenyl-2',3'-isopropyl-5'-UMP + H2O | - |
Rattus norvegicus | p-nitrophenol + isopropyl-5'-UMP | - |
? | |
polymer synthesized from NAD+ + H2O | chain length 27-30 | Rattus norvegicus | ? | - |
? | |
RNA + H2O | hydrolyzed very slowly | Rattus norvegicus | nucleoside 5'-phosphates | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
pH 7.3, 5 min, complete loss of activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.1 | - |
nitrophenyl uridine 5'-monophosphate | Rattus norvegicus |
10.5 | - |
NAD | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | nitrophenyl uridine 5'-monphosphate, pH 8: about 15% of maximum activity, pH 10: about 10% of maximum activity | Rattus norvegicus |