Literature summary for 3.1.13.2 extracted from

Rosen, L.E.; Kathuria, S.V.; Matthews, C.R.; Bilsel, O.; Marqusee, S.
Non-native structure appears in microseconds during the folding of E. coli RNase H (2015), J. Mol. Biol., 427, 443-453.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta2(DE3)pLysS cells	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
I53D	inactive	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
DNA-RNA hybrid + H2O	Escherichia coli	-	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A7Y4	-	-

Purification (Commentary)

Purification (Comment)	Organism
heparin column chromatography and HiTrap Capto S column chromatography	Escherichia coli

Renatured (Commentary)

Renatured (Comment)	Organism
using a 6 M to 0.6 M urea concentration jump	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA-RNA hybrid + H2O	-	Escherichia coli	?	-	?

Synonyms

Synonyms	Comment	Organism
RNase H	-	Escherichia coli

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Release 2023.1 (January 2023)