Protein Variants | Comment | Organism |
---|---|---|
Y5014-(phenylcarbonyl)-L-Phe | inserting a benzophenone into the RNase H primer grip (p66501BpF/p51 reverse transcriptase) creates an enzyme that is resistant to beta-thujaplicinol at inhibitor concentrations as high as 300 microM | Human immunodeficiency virus 1 |
Y5014-azido-L-Phe | nonnatural amino acid substitution introducing an azido function | Human immunodeficiency virus 1 |
Y501F | site-directed mutagenesis. IC50 for beta-thujaplicinol is comparable to wild-type value | Human immunodeficiency virus 1 |
Y501W | site-directed mutagenesis. IC50 for beta-thujaplicinol is comparable to wild-type value | Human immunodeficiency virus 1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-thujaplicinol | - |
Human immunodeficiency virus 1 | |
Phosphonoformic acid | inhibits both the polymerase and the RNase H activities | Human immunodeficiency virus 1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | binding of beta-thujaplicinol requires divalent metal ions | Human immunodeficiency virus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-RNA hybrid + H2O | - |
Human immunodeficiency virus 1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease H | - |
Human immunodeficiency virus 1 |
RNase H | HIV-1 reverse transcriptase possesses DNA polymerase and RNase H activities | Human immunodeficiency virus 1 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
Y5014-(phenylcarbonyl)-L-Phe mutant, the IC50 value is above 300 microM | Human immunodeficiency virus 1 | additional information | |
0.000308 | - |
wild-type | Human immunodeficiency virus 1 | beta-thujaplicinol | |
0.000356 | - |
Y501W mutant | Human immunodeficiency virus 1 | beta-thujaplicinol | |
0.000386 | - |
Y5014-azido-L-Phe mutant | Human immunodeficiency virus 1 | beta-thujaplicinol | |
0.00047 | - |
Y501F mutant | Human immunodeficiency virus 1 | beta-thujaplicinol |