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Literature summary for 3.1.13.2 extracted from
- Mutagenesis of Gln294 of the reverse transcriptase of human immunodeficiency virus type-2 and its effects on the ribonuclease H activity (2008), FEBS Lett., 582, 2799-2805.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli with 6-histidine tags | Human immunodeficiency virus 1 |
| expressed in Escherichia coli with 6-histidine tags | Human immunodeficiency virus 2 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | mutagenesis of Gln294 of the reverse transcriptase to find out how various amino acids, other that Gln and Pro at position 294 of HIV-2 reverse transcriptase affect the enzyme's RNase H activity. All modifications of HIV-2 reverse transcriptase Gln294 lead to an increase of the RNase H activity of the enzyme relative to the wild-type and also support the observations that residue 294 in the p54 subunit is the major contributor to the level of this activity in HIV-2 reverse transcriptase | Human immunodeficiency virus 2 |
| Q294A | 6.6fold RNase H activity compared to wild-type | Human immunodeficiency virus 2 |
| Q294C | 9.2fold RNase H activity compared to wild-type | Human immunodeficiency virus 2 |
| Q294E | 4.0fold RNase H activity compared to wild-type | Human immunodeficiency virus 2 |
| Q294H | 4.2fold RNase H activity compared to wild-type | Human immunodeficiency virus 2 |
| Q294M | 4.3fold RNase H activity compared to wild-type | Human immunodeficiency virus 2 |
| Q294N | 4.9fold RNase H activity compared to wild-type | Human immunodeficiency virus 2 |
| Q294P | 5.1fold RNase H activity compared to wild-type | Human immunodeficiency virus 2 |
| Q294Q/Q294R | 4.2fold RNase H activity compared to wild-type, Gln294 is modified in p54subunit, Q in p68, R in p54 | Human immunodeficiency virus 2 |
| Q294R | 4.8fold RNase H activity compared to wild-type | Human immunodeficiency virus 2 |
| Q294S | 6.9fold RNase H activity compared to wild-type | Human immunodeficiency virus 2 |
| Q294W | 5.1fold RNase H activity compared to wild-type | Human immunodeficiency virus 2 |
| Q294Y | 4.8fold RNase H activity compared to wild-type | Human immunodeficiency virus 2 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Human immunodeficiency virus 1 | RNA cleavage in the RNA-DNA hybrids by the various mutant HIV-2 reverse transcriptases, in comparison to the wild-type HIV-1 and HIV-2 reverse transcriptases | ? | - |
? |  |
| additional information | Human immunodeficiency virus 2 | RNA cleavage in the RNA-DNA hybrids by the various mutant HIV-2 reverse transcriptases, in comparison to the wild-type HIV-1 and HIV-2 reverse transcriptases | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Human immunodeficiency virus 1 | - |
- |
- |
| Human immunodeficiency virus 2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography using Ni-Agarose to enrich for the histidines-tagged proteins and the reverse transcriptase-containing fractions are further purified by carboxyl-methyl Sepharose ion exchange chromatography | Human immunodeficiency virus 1 |
| affinity chromatography using Ni-Agarose to enrich for the histidines-tagged proteins and the reverse transcriptase-containing fractions are further purified by carboxyl-methyl Sepharose ion exchange chromatography | Human immunodeficiency virus 2 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | RNA cleavage in the RNA-DNA hybrids by the various mutant HIV-2 reverse transcriptases, in comparison to the wild-type HIV-1 and HIV-2 reverse transcriptases | Human immunodeficiency virus 1 | ? | - |
? | |
| additional information | RNA cleavage in the RNA-DNA hybrids by the various mutant HIV-2 reverse transcriptases, in comparison to the wild-type HIV-1 and HIV-2 reverse transcriptases | Human immunodeficiency virus 2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | - |
Human immunodeficiency virus 2 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ribonuclease H | - |
Human immunodeficiency virus 1 |
| ribonuclease H | - |
Human immunodeficiency virus 2 |
| RNase H | reverse transcriptase domain | Human immunodeficiency virus 1 |
| RNase H | reverse transcriptase domain | Human immunodeficiency virus 2 |
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