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Literature summary for 3.1.13.2 extracted from

  • Pena, V.; Rozov, A.; Fabrizio, P.; Luehrmann, R.; Wahl, M.C.
    Structure and function of an RNase H domain at the heart of the spliceosome (2008), EMBO J., 27, 2929-2940.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.13.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D1853A failure of D1853A and R1937A mutants of scPrp8 to grow on 5-fluoroorotic acid plates, lethal mutation Saccharomyces cerevisiae
D1853N cell viability assay monitoring the effects of exchanging invariant Prp8 residues D1853, D1854, T1855, T1936 and R1937 as indicated. After selection of clones, the culture and serial dilutions are spotted and grown at the temperatures 16, 25, 30, and 37°C for 2 days Saccharomyces cerevisiae
D1854A cell viability assay monitoring the effects of exchanging invariant Prp8 residues D1853, D1854, T1855, T1936 and R1937 as indicated. After selection of clones, the culture and serial dilutions are spotted and grown at the temperatures 16, 25, 30, and 37°C for 2 days Saccharomyces cerevisiae
D1854N cell viability assay monitoring the effects of exchanging invariant Prp8 residues D1853, D1854, T1855, T1936 and R1937 as indicated. After selection of clones, the culture and serial dilutions are spotted and grown at the temperatures 16, 25, 30, and 37°C for 2 days Saccharomyces cerevisiae
E1960K mutant of prp8-101 Saccharomyces cerevisiae
K1864E prp8-D143, that suppress second-step defects in the 5'splice site, 3'splice site or branch point sequence map to the present Prp8 region Saccharomyces cerevisiae
additional information all mutants are associated with dramatic growth defects and produced both cold- and temperature-sensitive phenotypes on mutation to asparagine Saccharomyces cerevisiae
N1869D prp8-151, that suppress second-step defects in the 5'splice site, 3'splice site or branch point sequence map to the present Prp8 region Saccharomyces cerevisiae
N1869D/S1970R prp8-152, that suppress second-step defects in the 5'splice site, 3'splice site or branch point sequence map to the present Prp8 region Saccharomyces cerevisiae
R1922G prp8-D104 Saccharomyces cerevisiae
R1937A failure of D1853A and R1937A mutants of scPrp8 to grow on 5-fluoroorotic acid plates, lethal mutation Saccharomyces cerevisiae
R1937K cell viability assay monitoring the effects of exchanging invariant Prp8 residues D1853, D1854, T1855, T1936 and R1937 as indicated. After selection of clones, the culture and serial dilutions are spotted and grown at the temperatures 16, 25, 30, and 37°C for 2 days Saccharomyces cerevisiae
T1855A cell viability assay monitoring the effects of exchanging invariant Prp8 residues D1853, D1854, T1855, T1936 and R1937 as indicated. After selection of clones, the culture and serial dilutions are spotted and grown at the temperatures 16, 25, 30, and 37°C for 2 days Saccharomyces cerevisiae
T1936A cell viability assay monitoring the effects of exchanging invariant Prp8 residues D1853, D1854, T1855, T1936 and R1937 as indicated. After selection of clones, the culture and serial dilutions are spotted and grown at the temperatures 16, 25, 30, and 37°C for 2 days Saccharomyces cerevisiae
T1982A prp8-153, that suppress second-step defects in the 5'splice site, 3'splice site or branch point sequence map to the present Prp8 region Saccharomyces cerevisiae
T1982A, SA1966/7AG prp8-154, that suppress second-step defects in the 5'splice site, 3'splice site or branch point sequence map to the present Prp8 region Saccharomyces cerevisiae
T1982A,V1987A prp8-155, that suppress second-step defects in the 5'splice site, 3'splice site or branch point sequence map to the present Prp8 region Saccharomyces cerevisiae
V1870N prp8-162, that suppress second-step defects in the 5'splice site, 3'splice site or branch point sequence map to the present Prp8 region Saccharomyces cerevisiae
V1946A prp8-D103 Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P33334
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Prp8
-
 Saccharomyces cerevisiae
RNase H domain of pre-mRNA-splicing factor 8, scPrp8 Saccharomyces cerevisiae