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Literature summary for 3.1.13.2 extracted from

  • Mbisa, J.L.; Nikolenko, G.N.; Pathak, V.K.
    Mutations in the RNase H primer grip domain of murine leukemia virus reverse transcriptase decrease efficiency and accuracy of plus-strand DNA transfer (2005), J. Virol., 79, 419-427.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.13.2

Protein Variants

Protein Variants Comment Organism
A558V site-directed mutagenesis, the mutation in the enzyme primer grip results in about 3fold increased viral mutation rates compared to the wild-type enzyme/virus, reduced titer murine leukemia virus
Q559L site-directed mutagenesis, the mutation in the enzyme primer grip results in about 3fold increased viral mutation rates compared to the wild-type enzyme/virus murine leukemia virus
S557A site-directed mutagenesis, the mutation in the enzyme primer grip results in about 3fold increased viral mutation rates compared to the wild-type enzyme/virus, reduced titer murine leukemia virus
T590A site-directed mutagenesis, the mutation in the enzyme primer grip results in similar viral mutation rates compared to the wild-type enzyme/virus murine leukemia virus
Y586A site-directed mutagenesis, the mutant virus is not stable and cannot be expressed, no colonies murine leukemia virus
Y586F site-directed mutagenesis, the mutation in the enzyme primer grip results in 17fold increased viral mutation rates compared to the wild-type enzyme/virus murine leukemia virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA-RNA hybrid + H2O murine leukemia virus the RNase H primer grip is important for viral replication and replication fidelity, mutational analysis, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
murine leukemia virus
-
 i.e. MLV
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA-RNA hybrid + H2O the RNase H primer grip is important for viral replication and replication fidelity, mutational analysis, overview murine leukemia virus ?
-
 ?
DNA-RNA hybrid + H2O the RNase H catalytic efficiency and specificity is influences by the RNase H primer grip contacting the DNA primer strand and positioning the template strand near the RNase H active site, overview murine leukemia virus ?
-
 ?

Synonyms

Synonyms Comment Organism
More the enzyme is part of the viral reverse transcriptase murine leukemia virus
ribonuclease H
-
 murine leukemia virus
RNase H
-
 murine leukemia virus