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Literature summary for 3.1.13.2 extracted from
- Evidence that HIV-1 reverse transcriptase employs the DNA 3' end-directed primary/secondary RNase H cleavage mechanism during synthesis and strand transfer (2005), J. Biol. Chem., 280, 40534-40543.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged wild-type and mutant reverse transcriptases in Escherichia coli | Human immunodeficiency virus 1 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H539D | site-directed mutagenesis, the mutant enzyme shows an altered cleavage pattern compared to the wild-type enzyme, the mutant is inhibited in performing secondary cleavage directed by the 3' end primer independent of the presence of a hairpin structure or the need to carry out strand displacement | Human immunodeficiency virus 1 |
| H539F | site-directed mutagenesis, the mutant enzyme shows an altered cleavage pattern compared to the wild-type enzyme, the mutant is inhibited in performing secondary cleavage directed by the 3' end primer independent of the presence of a hairpin structure or the need to carry out strand displacement | Human immunodeficiency virus 1 |
| H539R | site-directed mutagenesis, the mutant enzyme shows an altered cleavage pattern compared to the wild-type enzyme, the mutant is inhibited in performing secondary cleavage directed by the 3' end primer independent of the presence of a hairpin structure or the need to carry out strand displacement | Human immunodeficiency virus 1 |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Human immunodeficiency virus 1 |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA-RNA hybrid + H2O | Human immunodeficiency virus 1 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Human immunodeficiency virus 1 | - |
i.e. HIV-1 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA-RNA hybrid + H2O | - |
Human immunodeficiency virus 1 | ? | - |
? | |
| DNA-RNA hybrid + H2O | cleavage pattern to 3' end of wild-type and mutant reverse transcriptase/RNase H, HIV-1 reverse transcriptase employs the DNA 3' end-directed primary/secondary RNase H cleavage mechanism during synthesis and strand transfer | Human immunodeficiency virus 1 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme is part of the viral reverse transcriptase | Human immunodeficiency virus 1 |
| ribonuclease H | - |
Human immunodeficiency virus 1 |
| RNase H | - |
Human immunodeficiency virus 1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Human immunodeficiency virus 1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Human immunodeficiency virus 1 |
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