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Literature summary for 3.1.13.2 extracted from

  • Sevilya, Z.; Loya, S.; Duvshani, A.; Adir, N.; Hizi, A.
    Mutagenesis of cysteine 280 of the reverse transcriptase of human immunodeficiency virus type-1: The effects on the ribonuclease H activity (2003), J. Mol. Biol., 327, 19-30.
    View publication on PubMed
Search for more literature for 3.1.13.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Human immunodeficiency virus 1

Protein Variants

Protein Variants Comment Organism
C280E mutation does not affect RNase H activity Human immunodeficiency virus 1
C280H mutation does not affect RNase H activity Human immunodeficiency virus 1
C280L mutation does not affect RNase H activity Human immunodeficiency virus 1
C280M mutation does not affect RNase H activity Human immunodeficiency virus 1
C280P mutation in either p66 or p51 subunits reduces RNase H activity in comparison to wild-type enzyme, the strand-transfer activity is very low Human immunodeficiency virus 1
C280Q mutation does not affect RNase H activity Human immunodeficiency virus 1
C280R mutation does not affect RNase H activity Human immunodeficiency virus 1
C280W mutation in either p66 or p51 subunits reduces RNase H activity in comparison to wild-type enzyme, the strand-transfer activity is very low Human immunodeficiency virus 1
C280Y mutation does not affect RNase H activity Human immunodeficiency virus 1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 poly(rA)n-poly(dT)n wild type enzyme p66/p51 Human immunodeficiency virus 1
0.018
-
 poly(rA)n-poly(dT)n mutant p66C280P/p51 Human immunodeficiency virus 1
0.026
-
 poly(rA)n-poly(dT)n mutant p66/p51C280P Human immunodeficiency virus 1
0.032
-
 poly(rA)n-poly(dT)n mutant p66C280W/p51 Human immunodeficiency virus 1
0.038
-
 poly(rA)n-poly(dT)n mutant p66/p51C280W Human immunodeficiency virus 1
0.055
-
 poly(rA)n-poly(dT)n mutant p66C280P/p51C280P Human immunodeficiency virus 1
0.066
-
 poly(rA)n-poly(dT)n mutant p66C280W/p51C280W Human immunodeficiency virus 1

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1
-
 HIV-1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information the chemical modification by thiol-specific reagents of cysteine 280, located in alpha helix I in the thumb subdomain of the polymerase domain, affect substantially only the RNase activity Human immunodeficiency virus 1

Reaction

Reaction Comment Organism Reaction ID
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid the enzyme reverse transcriptase has polymerase and RNase H activities Human immunodeficiency virus 1
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 comparison of relative activities of wild-type and mutant enzymes Human immunodeficiency virus 1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(rA)n-poly(dT)n
-
 Human immunodeficiency virus 1 ?
-
 ?
poly(rA)n-poly(dT)ne + H2O
-
 Human immunodeficiency virus 1 ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer the smaller subunit p51 and the larger subunit p66 Human immunodeficiency virus 1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0024
-
 poly(rA)n-poly(dT)n mutant p66/p51C280W Human immunodeficiency virus 1
0.0024
-
 poly(rA)n-poly(dT)ne mutant p66C280P/p51 Human immunodeficiency virus 1
0.0025
-
 poly(rA)n-poly(dT)n mutant p66/p51C280P Human immunodeficiency virus 1
0.0029
-
 poly(rA)n-poly(dT)n mutant p66C280P/p51C280P Human immunodeficiency virus 1
0.0029
-
 poly(rA)n-poly(dT)n mutant p66C280W/p51 Human immunodeficiency virus 1
0.0031
-
 poly(rA)n-poly(dT)n wild type enzyme p66/p51 Human immunodeficiency virus 1
0.0034
-
 poly(rA)n-poly(dT)n mutant p66C280W/p51C280W Human immunodeficiency virus 1