Application | Comment | Organism |
---|---|---|
medicine | the enzyme is a major target for the chemotherapy of human immunodeficiency syndrome, AIDS | Human immunodeficiency virus 1 |
medicine | the enzyme is a major target for the chemotherapy of human immunodeficiency syndrome, AIDS | Human immunodeficiency virus 2 |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Human immunodeficiency virus 1 |
expression in Escherichia coli and expression in bacteria by plasmids | Human immunodeficiency virus 2 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | - |
HIV-1 | - |
Human immunodeficiency virus 2 | - |
Rod strain and D-194 strain of HIV-2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | construction of chimeric HIV-1/HIV-2 enzymes, The specific activity is either high, like HIV-1 enzyme, or low, like HIV-2 enzyme. The origin of the thumb subdomain in the small subunit of the chimeric enzymes, residues 244-322, determines the level of the activity. | Human immunodeficiency virus 1 |
additional information | construction of chimeric HIV-1/HIV-2 enzymes. The specific activity is either high, like HIV-1 enzyme, or low, like HIV-2 enzyme. The origin of the thumb subdomain in the small subunit of the chimeric enzymes, residues 244-322, determines the level of the activity. | Human immunodeficiency virus 2 |
Purification (Comment) | Organism |
---|---|
wild-type and chimeric enzymes | Human immunodeficiency virus 1 |
wild-type and chimeric enzymes | Human immunodeficiency virus 2 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
comparison of relative activities of wild-type and chimeric enzymes, the enzyme activity of HIV-2 is about tenfold lower than of HIV-1 | Human immunodeficiency virus 1 |
additional information | - |
comparison of relative activities of wild-type and chimeric enzymes, the enzyme activity of HIV-2 is about tenfold lower than of HIV-1 | Human immunodeficiency virus 2 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5' end-labeled RNA | cleavage of 267 nt RNA substrate to produce a 47 nt long product, the second cleavage produces RNA fragments that are 38 nt long | Human immunodeficiency virus 1 | RNA fragments | - |
? | |
5'-end-labeled RNA | cleavage of 267nt RNA substrate to produce a 47nt long product, the second cleavage produces RNA fragments that are 38nt long. | Human immunodeficiency virus 2 | RNA fragments | - |
? | |
5'-end-labeled RNA | cleavage of 267nt RNA substrate to produce a 47nt long product, the second cleavage produces RNA fragments that are 38nt long. | Human immunodeficiency virus 2 D-194 | RNA fragments | - |
? | |
5'-end-labeled RNA | cleavage of 267nt RNA substrate to produce a 47nt long product, the second cleavage produces RNA fragments that are 38nt long. | Human immunodeficiency virus 2 ROD | RNA fragments | - |
? | |
poly(rA)n-poly(dT)n | - |
Human immunodeficiency virus 1 | ? | - |
? | |
poly(rA)n-poly(dT)n | - |
Human immunodeficiency virus 2 | ? | - |
? | |
poly(rA)n-poly(dT)n | - |
Human immunodeficiency virus 2 D-194 | ? | - |
? | |
poly(rA)n-poly(dT)n | - |
Human immunodeficiency virus 2 ROD | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | p66/p51 | Human immunodeficiency virus 1 |
heterodimer | p68/p54 | Human immunodeficiency virus 2 |