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Literature summary for 3.1.13.1 extracted from

  • Keir, H.M.; Mathog, R.H.; Carter, C.E.
    Purification of a potassium ion-activated RNA, 5'-phosphodiesterase from Lactobacillus casei (1964), Biochemistry, 3, 1188-1193.
    View publication on PubMed
Search for more literature for 3.1.13.1

Application

Application Comment Organism
analysis
-
 Lacticaseibacillus casei

Localization

Localization Comment Organism GeneOntology No. Textmining
ribosome
-
 Lacticaseibacillus casei 5840
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activating Lacticaseibacillus casei
K+ maximum concentration for activation: 0.15 mM at pH 7.4 and 8.0 Lacticaseibacillus casei
NH4+ can substitute for K+ to a small extend Lacticaseibacillus casei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ss RNA + H2O Lacticaseibacillus casei
-
 5'-phosphomononucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus casei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
28.6fold Lacticaseibacillus casei

Reaction

Reaction Comment Organism Reaction ID
Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates can act as a endonuclease too Lacticaseibacillus casei
a

Storage Stability

Storage Stability Organism
-10°C, 1.5 ml aliquots, 1 mg of protein per ml, 6 months, small decrease of activity Lacticaseibacillus casei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ss RNA + H2O
-
 Lacticaseibacillus casei 5'-phosphomononucleotides
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 minor pH optimum Lacticaseibacillus casei
8 8.2
-
 Lacticaseibacillus casei