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Literature summary for 3.1.12.1 extracted from

  • Kaushik, V.; Verma, V.V.; Goel, M.
    Functional divergence and comparative in-silico study of Cas4 proteins of DUF83 class (2018), J. Mol. Recognit., 31, e2694 .
    View publication on PubMed
Search for more literature for 3.1.12.1

Cloned(Commentary)

Cloned (Comment) Organism
gene Pcal_0546, phylogenetic analysis and tree Pyrobaculum calidifontis
gene SSO0001, phylogenetic analysis and tree Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Pyrobaculum calidifontis A3MTK6
-
-
Pyrobaculum calidifontis VA1 A3MTK6
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-
Saccharolobus solfataricus Q97TX9 i.e. Sulfolobus solfataricus
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Saccharolobus solfataricus ATCC 35092 Q97TX9 i.e. Sulfolobus solfataricus
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Saccharolobus solfataricus DSM 1617 Q97TX9 i.e. Sulfolobus solfataricus
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Saccharolobus solfataricus JCM 11322 Q97TX9 i.e. Sulfolobus solfataricus
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Saccharolobus solfataricus P2 Q97TX9 i.e. Sulfolobus solfataricus
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Synonyms

Synonyms Comment Organism
Cas4
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 Saccharolobus solfataricus
Cas4
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 Pyrobaculum calidifontis
CRISPR-associated exonuclease Cas4
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 Saccharolobus solfataricus
CRISPR-associated exonuclease Cas4
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 Pyrobaculum calidifontis
DUF83Pc
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 Pyrobaculum calidifontis
DUF83Ss
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 Saccharolobus solfataricus
Pcal_0546
-
 Pyrobaculum calidifontis
SSO0001
-
 Saccharolobus solfataricus

General Information

General Information Comment Organism
evolution Cas4 proteins exhibit low sequence similarity among themselves and are currently classified into 2 main classes: DUF83 and DUF911. The genome of Sulfolobus solfataricus encodes five Cas4 like protein comprising low sequence similarity (below 30%). Out of them, SSO0001, SSO1392, and SSO1449 belong to DUF83 class, and SSO1391 and SSO1451 belong to DUF911 class. SSO1391 has been reported to show broad range cleavage specificity for cleaving ssDNA in both the directions (5'-3') and (3'-5') (DUF911). By contrast, members of DUF83 class cleave the ssDNA substrate only in the direction 5'-3'. The overall three-dimensional structural fold of DUF83Ss and DUF83Pc appears to be conserved. The separation of Cas4 proteins of DUF83 class into 2 separate branches hints at the possibility of specific sites which guide the evolutionary divergence between Cas4 proteins of the 2 clades, prediction of discriminative motifs among the two subclasses of DUF83 Cas4 proteins through motif analysis, overview. Divergence type I analyses of two clusters and their intra molecular hydrogen bonding with other residues Saccharolobus solfataricus
evolution Cas4 proteins exhibit low sequence similarity among themselves and are currently classified into 2 main classes: DUF83 and DUF911. The genome of Sulfolobus solfataricus encodes five Cas4 like protein comprising low sequence similarity (below 30%). Out of them, SSO0001, SSO1392, and SSO1449 belong to DUF83 class, and SSO1391 and SSO1451 belong to DUF911 class. SSO1391 has been reported to show broad range cleavage specificity for cleaving ssDNA in both the directions (5'-3') and (3'-5') (DUF911). By contrast, members of DUF83 class cleave the ssDNA substrate only in the direction 5'-3'. The overall three-dimensional structural fold of DUF83Ss and DUF83Pc appears to be conserved. The separation of Cas4 proteins of DUF83 class into 2 separate branches hints at the possibility of specific sites which guide the evolutionary divergence between Cas4 proteins of the 2 clades, prediction of discriminative motifs among the two subclasses of DUF83 Cas4 proteins through motif analysis, overview. Divergence type I analyses of two clusters and their intra molecular hydrogen bonding with other residues Pyrobaculum calidifontis
physiological function the Cas4 proteins of the CRISPR-Cas systems are thought to be responsible for the adaptation step in the CRISPR (clustered regularly interspaced short palindromic repeats) mechanism Saccharolobus solfataricus
physiological function the Cas4 proteins of the CRISPR-Cas systems are thought to be responsible for the adaptation step in the CRISPR (clustered regularly interspaced short palindromic repeats) mechanism Pyrobaculum calidifontis