additional information |
effect of xonA (ExoI), xseA (ExoVII) and sbcCD (SbcCD) mutations on growth without DnaA (dnaA46 at 42°C). The replication fork trap in the termination area is inactivated by deletion of tus and an rpoB*35 point mutation is introduced to alleviate replication-transcription conflicts resulting from replication forks travelling in a direction opposite to normal. The DnaA-independent growth is triggered by the absence of 3' exonucleases. Generation of RCe267 (dnaA46 DELTAtus rpo*), RCe528 (dnaA46 DELTAtus rpo* DELTAxonA), SLM1219 (dnaA46 DELTAtus rpo* DELTAxseA), RCe553 (dnaA46 DELTAtus rpo* DELTAsbcCD), SLM1194 (dnaA46 DELTAtus rpo* DELTAxonA* DELTAxseA), RCe554 (dnaA46 DELTAtus rpo* DELTAxonA DELTAsbcCD), SLM1223 (dnaA46 DELTAtus rpo* DELTAxseA DELTAsbcCD) and SLM1226 (dnaA46 DELTAtus rpo* DELTAxonA DELTAxseA DELTAsbcCD) mutant strains, phenotypes, overview. Overreplication of the termination area in cells lacking 3' exonucleases is abolished if the chromosome is linearized. And overreplication of the termination area in cells lacking 3' exonucleases requires PriA helicase activity. An asymmetric replichore arrangement does not increase overreplication of the termination area in cells lacking 3' exonucleases |
Escherichia coli |