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Literature summary for 3.1.11.5 extracted from

  • Kushner, S.R.
    Differential thermolability of exonuclease and endonuclease activities of the recBC nuclease isolated from thermosensitive recB and recC mutants (1974), J. Bacteriol., 120, 1219-1222.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis possible role in genetic recombination Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + H2O Escherichia coli responsible for several steps in genetic recombination process single-stranded DNA fragments
-
?
double-stranded DNA + H2O Escherichia coli ATP-dependent exonuclease single-stranded DNA fragments
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
thermosensitive mutants with thermolabile adenosine 5'-triphosphate-dependent exonucleolytic hydrolysis of duplex DNA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded DNA + H2O only ATP-dependent exonuclease activity is altered in thermolabile recB and recC mutants Escherichia coli single-stranded DNA fragments
-
?
double-stranded DNA + H2O responsible for several steps in genetic recombination process Escherichia coli single-stranded DNA fragments
-
?
double-stranded DNA + H2O ATP-dependent exonuclease Escherichia coli single-stranded DNA fragments
-
?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 43 pH 9.0, wild type, at 43°C fourfold higher activity than at 30°C Escherichia coli