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Literature summary for 3.1.11.5 extracted from

  • Goldmark, P.J.; Linn, S.
    Purification and properties of the recBC DNase of Escherichia coli K-12 (1972), J. Biol. Chem., 247, 1849-1860.
    View publication on PubMed
Search for more literature for 3.1.11.5

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin 2-3fold stimulation Escherichia coli
bovine serum albumin required for endonuclaes activity Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
128000
-
 1 * 140000 + 1 * 128000, dissociation by boiling, SDS-PAGE Escherichia coli
140000
-
 1 * 140000 + 1 * 128000, dissociation by boiling, SDS-PAGE Escherichia coli
270000
-
 gel filtration, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + H2O Escherichia coli ATP-dependent exonuclease single-stranded DNA fragments
-
 ?
single stranded DNA + H2O Escherichia coli ATP-dependent endonuclease 5'-phosphomonoester oligonucleotides
-
 ?
single stranded DNA + H2O Escherichia coli ATP-stimulated endonuclease 5'-phosphomonoester oligonucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded DNA + H2O
-
 Escherichia coli single-stranded DNA fragments
-
 ?
double-stranded DNA + H2O ATP-dependent exonuclease Escherichia coli single-stranded DNA fragments
-
 ?
single stranded DNA + H2O also endonuclease activity on circular DNA Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?
single stranded DNA + H2O ATP-dependent endonuclease Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?
single stranded DNA + H2O ATP-stimulated endonuclease Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 140000 + 1 * 128000, dissociation by boiling, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 endonuclease activity Escherichia coli
9
-
 exonuclease activity Escherichia coli