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Literature summary for 3.1.11.3 extracted from

  • Foulk, M.; Urban, J.; Casella, C.; Gerbi, S.
    Characterizing and controlling intrinsic biases of lambda exonuclease in nascent strand sequencing reveals phasing between nucleosomes and G-quadruplex motifs around a subset of human replication origins (2015), Genome Res., 25, 725-735 .
No PubMed abstract available
Search for more literature for 3.1.11.3

Application

Application Comment Organism
molecular biology nascent strand sequencing (NS-seq) is used to discover DNA replication origins genome-wide, allowing identification of features for their specification. NS-seq depends on the ability of lambda exonuclease (lambda-exo) to efficiently digest parental DNA while leaving RNA-primer protected nascent strands intact. LexoG0 data to control for nascent strand-independent lambda-exo biases in NSseq and validated this approach at the rDNA locus. Possible increase of both sensitivity and specificity of NS-seq, overview Lambdavirus lambda

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Lambdavirus lambda G-quadruplexes are resistant to lambda-exo digestion ?
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 ?

Organism

Organism UniProt Comment Textmining
Lambdavirus lambda P03697
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information G-quadruplexes are resistant to lambda-exo digestion Lambdavirus lambda ?
-
 ?
additional information analysis of the ability of enzyme lambda-exo to efficiently digest G4 structures by digesting a plasmid derived from the human MYC locus (from MCF-7 breast cancer cells) that contains a well-characterized G4 motif, overview. Genome-wide biases of lambda-exo are profiled by sequencing the DNA remaining after lambda-exo digestion of sonicated genomic DNA (gDNA) from nonreplicating G0 MCF7 cells (LexoG0). The enzyme does not efficiently digest G-quadruplex (G4) structures in a plasmid. And enzyme lambda-exo digestion of nonreplicating genomic DNA (LexoG0) enriches GC-rich DNA and G4 motifs genome-wide Lambdavirus lambda ?
-
 ?

Synonyms

Synonyms Comment Organism
lambda exonuclease
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 Lambdavirus lambda
lambda-exo
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 Lambdavirus lambda

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Lambdavirus lambda

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 30 min, pH 9.4, inactivation Lambdavirus lambda

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.4
-
 assay at Lambdavirus lambda

General Information

General Information Comment Organism
physiological function analysis of intrinsic biases of lambda exonuclease in nascent strand sequencing reveals phasing between nucleosomes and G-quadruplex motifs around a subset of human replication origins Lambdavirus lambda