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Literature summary for 3.1.11.3 extracted from
- Sensitive discrimination of stable mismatched base pairs by an abasic site modified fluorescent probe and lambda exonuclease (2015), Chem. Commun. (Camb.), 51, 17402-17405 .
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Lambdavirus lambda |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lambdavirus lambda | P03697 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATGTGTCTGTGVAGACGAGAGTAAG + H2O | a target sequence | Lambdavirus lambda | ? | - |
? | |
| GTGTCTGTGVAGACGAGAGTAAG + H2O | a target sequence | Lambdavirus lambda | ? | - |
? | |
| GTTTTAAATTATGGAGTATGTCAVTGTGGAGACGAGAGTAAG + H2O | a target sequence | Lambdavirus lambda | ? | - |
? | |
| GTTTTAAATTATGGAGTATGTGAVTGTGGAGACGAGAGTAAG + H2O | a target sequence | Lambdavirus lambda | ? | - |
? | |
| GTTTTAAATTATGGAGTATGTGTCTGTGCAVACGAGAGTAAG + H2O | a target sequence | Lambdavirus lambda | ? | - |
? | |
| GTTTTAAATTATGGAGTATGTGTCTGTGGAVACGAGAGTAAG + H2O | a target sequence | Lambdavirus lambda | ? | - |
? | |
| GTTTTAAATTATGGAGTATGTGTCTGTGVAGACGAGAGTAAG + H2O | a target sequence | Lambdavirus lambda | ? | - |
? | |
| additional information | sensitive discrimination of stable mismatched base pairs by an abasic site modified fluorescent probe and lambda exonuclease, substrate is target ssDNA with diverse DNA sequences, overview. Substrate specificity for base positions, discrimination factors of C:C type mismatched probe/target duplex, discrimination factors (DF) for duplexes containing different types of mismatched base pairs at position +I by using the abasic site modified probe in the absence and presence of different blocker strands, tested blocker sequences for the targets and type of mismatched base pair, overview | Lambdavirus lambda | ? | - |
? | |
| PO4-TAT(-FAM)CCACAGACACATACTCCA-BHQ1 + H2O | a probe sequence | Lambdavirus lambda | ? | - |
? | |
| PO4-TCTCCACAYT(-FAM)CACATACTCCA-BHQ1 + H2O | a probe sequence, Y = tetrahydrofuran abasic site | Lambdavirus lambda | ? | - |
? | |
| PO4-TUT(-FAM)ACACAGACACATACTCCA-BHQ1 + H2O | a probe sequence | Lambdavirus lambda | ? | - |
? | |
| PO4-TUT(-FAM)TCACAGACACATACTCCA-BHQ1 + H2O | a probe sequence | Lambdavirus lambda | ? | - |
? | |
| PO4-TXT(-FAM)TCACAGACACATACTCCA-BHQ1 + H2O | a probe sequence, X = natural abasic site generated by UDG | Lambdavirus lambda | ? | - |
? | |
| PO4-TYT(-FAM)ACACAGACACATACTCCA-BHQ1 + H2O | a probe sequence, Y = tetrahydrofuran abasic site | Lambdavirus lambda | ? | - |
? | |
| PO4-TYT(-FAM)CCACAGACACATACTCCA-BHQ1 + H2O | a probe sequence, Y = tetrahydrofuran abasic site | Lambdavirus lambda | ? | - |
? | |
| PO4-TYT(-FAM)TCACAGACACATACTCCA-BHQ1 + H2O | a probe sequence, Y = tetrahydrofuran abasic site | Lambdavirus lambda | ? | - |
? | |
| PO4-TYTCCACAGACACAT(-FAM)ACTCCATAATTTAA-BHQ1 + H2O | a probe sequence, Y = tetrahydrofuran abasic site | Lambdavirus lambda | ? | - |
? | |
| TATGTGTCTGTGVAGACGAGAGTAAG + H2O | a target sequence | Lambdavirus lambda | ? | - |
? | |
| TGTGTCTGTGVAGACGAGAGTAAG + H2O | a target sequence | Lambdavirus lambda | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lambda exonuclease | - |
Lambdavirus lambda |
| lambda-exo | - |
Lambdavirus lambda |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Lambdavirus lambda |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.4 | - |
assay at | Lambdavirus lambda |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Lambdavirus lambda | |
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