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Literature summary for 3.1.11.3 extracted from

  • Yuan, Z.; Zhou, Y.; Gao, S.; Cheng, Y.; Li, Z.
    Homogeneous and sensitive detection of microRNA with ligase chain reaction and lambda exonuclease-assisted cationic conjugated polymer biosensing (2014), ACS Appl. Mater. Interfaces, 6, 6181-6185.
    View publication on PubMed

Application

Application Comment Organism
analysis simple and homogeneous microRNA assay by integration of ligase chain reaction and lambda exonuclease-assisted cationic conjugated polymer biosensing. Ligase chain reaction is utilized for exponential amplification of microRNA, and lambda exonuclease is introduced to degrade excess fluorescein-labeled probes in ligase chain reaction for eliminating background signal. The method is sensitive enough to detect 0.1 fM target microRNA and specific to discriminate one-base difference of microRNAs Lambdavirus lambda

Organism

Organism UniProt Comment Textmining
Lambdavirus lambda
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