Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
gel fitration, SDS-PAGE | Escherichia coli |
24000 | - |
gel fitration, SDS-PAGE | Lambdavirus lambda |
28000 | - |
alpha,beta, 1 * 24000 + 1 * 28000, exonuclease and beta protein as subunits, SDS-PAGE at the presence of urea | Escherichia coli |
28000 | - |
alpha,beta, 1 * 24000 + 1 * 28000, exonuclease and beta protein as subunits, SDS-PAGE at the presence of urea | Lambdavirus lambda |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | Escherichia coli | - |
single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
double-stranded DNA + H2O | Lambdavirus lambda | - |
single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
single-stranded DNA + H2O | Escherichia coli | - |
5'-phosphomononucleotides | - |
? | |
single-stranded DNA + H2O | Lambdavirus lambda | - |
5'-phosphomononucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
Escherichia coli infected with phage lambda only | - |
Lambdavirus lambda | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Lambdavirus lambda |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | - |
Escherichia coli | single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
double-stranded DNA + H2O | - |
Lambdavirus lambda | single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
double-stranded DNA + H2O | no attack at single-stranded breaks within DNA-duplex | Escherichia coli | single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
double-stranded DNA + H2O | no attack at single-stranded breaks within DNA-duplex | Lambdavirus lambda | single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
single-stranded DNA + H2O | - |
Escherichia coli | 5'-phosphomononucleotides | - |
? | |
single-stranded DNA + H2O | - |
Lambdavirus lambda | 5'-phosphomononucleotides | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha,beta, 1 * 24000 + 1 * 28000, exonuclease and beta protein as subunits, SDS-PAGE at the presence of urea | Escherichia coli |
dimer | alpha,beta, 1 * 24000 + 1 * 28000, exonuclease and beta protein as subunits, SDS-PAGE at the presence of urea | Lambdavirus lambda |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
no activity at DNA nicks | Escherichia coli |
7.5 | - |
no activity at DNA nicks | Lambdavirus lambda |
9.6 | - |
no activity at DNA nicks | Escherichia coli |
9.6 | - |
no activity at DNA nicks | Lambdavirus lambda |