Literature summary for 3.1.11.1 extracted from

Miyazono, K.I.; Tsutsumi, K.; Ishino, Y.; Tanokura, M.
Expression, high-pressure refolding, purification, crystallization and preliminary X-ray analysis of a novel single-strand-specific 3'-5' exonuclease PhoExo I from Pyrococcus horikoshii OT3 (2014), Acta Crystallogr. Sect. F, 70, 1076-1079.

Cloned(Commentary)

Cloned (Comment)	Organism
produced as inclusion bodies in Escherichia coli	Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment)	Organism
solubilization of the inclusion bodies is performed by the high-pressure refolding method and highly purified protein is subjected to crystallization by the sitting-drop vapour-diffusion method at 20°C. A crystal of PhoExo I is obtained in a reservoir solution consisting of 0.1 M Tris-HCl pH 8.9, 27% PEG 6000 and diffracts X-rays to 1.52 A resolution. The crystal of PhoExo I belong to space group H32, with unit-cell parameters a = b = 112.07, c = 202.28 A. The crystal contains two PhoExo I molecules in the asymmetric unit	Pyrococcus horikoshii

Crystallization (Comment)

Organism

solubilization of the inclusion bodies is performed by the high-pressure refolding method and highly purified protein is subjected to crystallization by the sitting-drop vapour-diffusion method at 20°C. A crystal of PhoExo I is obtained in a reservoir solution consisting of 0.1 M Tris-HCl pH 8.9, 27% PEG 6000 and diffracts X-rays to 1.52 A resolution. The crystal of PhoExo I belong to space group H32, with unit-cell parameters a = b = 112.07, c = 202.28 A. The crystal contains two PhoExo I molecules in the asymmetric unit

Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
75000	-	gel filtration	Pyrococcus horikoshii

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	-	-	-
Pyrococcus horikoshii DSM 12428	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5'-fluorescein-TTTTTTTTTTTTTTTTTTTTTTTTT-3' + H2O	-	Pyrococcus horikoshii	?	-	?
5'-fluorescein-TTTTTTTTTTTTTTTTTTTTTTTTT-3' + H2O	-	Pyrococcus horikoshii DSM 12428	?	-	?

Subunits

Subunits	Comment	Organism
homotrimer	-	Pyrococcus horikoshii

Synonyms

Synonyms	Comment	Organism
PhoExo I	-	Pyrococcus horikoshii
single-strand-specific 3'-5' exonuclease	-	Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	assay at	Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Pyrococcus horikoshii

General Information

General Information	Comment	Organism
physiological function	the enzyme is involved in a Thermococcales-specific DNA-repair pathway	Pyrococcus horikoshii