Protein Variants | Comment | Organism |
---|---|---|
D173A | site-directed mutagenesis, the exo1-D173A mutant defective in nuclease activity is able to maintain crossing-over at wild-type levels in a number of genetic intervals. Exo1-D173A cells are able to maintain crossing-over despite reducedhDNAformation | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | Exo1 possesses both 5'-3' exonuclease and 5' flap endonuclease activities | ? | - |
? | |
additional information | Saccharomyces cerevisiae SK1 | Exo1 possesses both 5'-3' exonuclease and 5' flap endonuclease activities | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
diverse genotypes, overview | - |
Saccharomyces cerevisiae SK1 | - |
diverse genotypes, overview | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Exo1 possesses both 5'-3' exonuclease and 5' flap endonuclease activities | Saccharomyces cerevisiae | ? | - |
? | |
additional information | Exo1 possesses both 5'-3' exonuclease and 5' flap endonuclease activities | Saccharomyces cerevisiae SK1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Exo1 | - |
Saccharomyces cerevisiae |
exonuclease I | - |
Saccharomyces cerevisiae |
More | Exo1 is a member of the Rad2 protein family | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
physiological function | in addition to performing a variety of functions during mitotic growth, Exo1 is also important for the production of crossovers during meiosis, the nuclease activity of exonuclease I is essential for normal 5'-3' resection at the Spo11-dependent HIS4 hotspot in otherwise wild-type cells. This same activity is also required for normal levels of gene conversion at the locus. Exo1 also plays a nuclease-independent role in crossover promotion | Saccharomyces cerevisiae |