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Literature summary for 3.1.11.1 extracted from

  • Orans, J.; McSweeney, E.A.; Iyer, R.R.; Hast, M.A.; Hellinga, H.W.; Modrich, P.; Beese, L.S.
    Structures of human exonuclease 1 DNA complexes suggest a unified mechanism for nuclease family (2011), Cell, 145, 212-223.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization of recombinant wild-type native and selenomethionine-labeled enzyme, and of recombinant mutant D173A enzyme in complex with metal ions, X-ray diffraction structure determination and analysis at 2.5-3.4 A resolution, modelling Homo sapiens

Protein Variants

Protein Variants Comment Organism
D173A inactive mutant, structure in complex with Ca2+, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ the K+-binding site lies in the H2TH domain Homo sapiens
Mg2+ required Homo sapiens
additional information wild-type enzyme structures in complex with Mn2+ or Ba2+, binding structures, anomalous scattering, overview Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UQ84
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate is radiolabeled 5' recessed DNA, interactions between the hExo1 protein and DNA substrate, overview Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
More enzyme structure analysis, including pre-nick and post-nick DNA-binding region, and the active site Homo sapiens

Synonyms

Synonyms Comment Organism
Exo1
-
Homo sapiens
exonuclease 1
-
Homo sapiens
More Exo1 is a member of the 5' structure-specific nuclease family of exonucleases and endonucleases that includes FEN-1, XPG, and GEN1 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Homo sapiens

General Information

General Information Comment Organism
additional information structures of hExo1 in complex with a DNA substrate, overview Homo sapiens
physiological function human exonuclease 1 plays important roles in DNA repair and recombination processes that maintain genomic integrity, model of 5' exonuclease activity in human DNA mismatch repair, overview. Analysis of the mechanism by which this 5' structure-specific exo- and endonuclease family, including Exo1, recognizes and processes diverse DNA structures, conformational control of a mobile region in the catalytic site suggests a mechanism for allosteric regulation by binding to protein partners, overview Homo sapiens