Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.11.1 extracted from

  • Shimada, A.; Masui, R.; Nakagawa, N.; Takahata, Y.; Kim, K.; Kuramitsu, S.; Fukui, K.
    A novel single-stranded DNA-specific 3'-5' exonuclease, Thermus thermophilus exonuclease I, is involved in several DNA repair pathways (2010), Nucleic Acids Res., 38, 5692-5705.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.11.1

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 ssDNA 50 mer pH 7.5, 37°C Thermus thermophilus
0.16
-
 ssDNA 50 mer pH 7.5, 60°C Thermus thermophilus
0.24
-
 ssDNA 40 mer pH 7.5, 37°C Thermus thermophilus
0.26
-
 ssDNA 28 mer pH 7.5, 37°C Thermus thermophilus
0.28
-
 ssDNA 10 mer pH 7.5, 37°C Thermus thermophilus
0.29
-
 ssDNA 28 mer pH 7.5, 60°C Thermus thermophilus
0.32
-
 ssDNA 40 mer pH 7.5, 60°C Thermus thermophilus
0.35
-
 ssDNA 21 mer pH 7.5, 37°C Thermus thermophilus
0.44
-
 ssDNA 21 mer pH 7.5, 60°C Thermus thermophilus
0.48
-
 ssDNA 10 mer pH 7.5, 60°C Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activity requires divalent cations Thermus thermophilus
Mg2+ activity requires divalent cations Thermus thermophilus
Mn2+ activity requires divalent cations Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ssDNA + H2O Thermus thermophilus TTHB178 possesses 3'–5'-ssExo activity that degrades ssDNAs containing deaminated and methylated bases, but not ssDNA containing oxidized bases or abasic sites. The enzyme functions in various DNA repair systems in cooperation with or independently of RecJ ?
-
 ?
ssDNA + H2O Thermus thermophilus HB8 / ATCC 27634 / DSM 579 TTHB178 possesses 3'–5'-ssExo activity that degrades ssDNAs containing deaminated and methylated bases, but not ssDNA containing oxidized bases or abasic sites. The enzyme functions in various DNA repair systems in cooperation with or independently of RecJ ?
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q53VZ1
-
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q53VZ1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity against ssRNA and dsDNA is observed Thermus thermophilus ?
-
 ?
additional information no activity against ssRNA and dsDNA is observed Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?
ssDNA + H2O TTHB178 possesses 3'–5'-ssExo activity that degrades ssDNAs containing deaminated and methylated bases, but not ssDNA containing oxidized bases or abasic sites. The enzyme functions in various DNA repair systems in cooperation with or independently of RecJ Thermus thermophilus ?
-
 ?
ssDNA + H2O TTHB178 possesses 3'–5'-ssExo activity that degrades ssDNAs containing deaminated and methylated bases, but not ssDNA containing oxidized bases or abasic sites. The enzyme functions in various DNA repair systems in cooperation with or independently of RecJ Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?
ssDNA 10 mer + H2O
-
 Thermus thermophilus ?
-
 ?
ssDNA 10 mer + H2O
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?
ssDNA 21 mer + H2O
-
 Thermus thermophilus ?
-
 ?
ssDNA 21 mer + H2O
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?
ssDNA 28 mer + H2O
-
 Thermus thermophilus ?
-
 ?
ssDNA 28 mer + H2O
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?
ssDNA 40 mer + H2O
-
 Thermus thermophilus ?
-
 ?
ssDNA 50 mer + H2O
-
 Thermus thermophilus ?
-
 ?

Synonyms

Synonyms Comment Organism
30–50 ssExo
-
 Thermus thermophilus
single-stranded DNA-specific 3'-5' exonuclease I
-
 Thermus thermophilus
tthb178
-
 Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 the kcat/KM value is 4-fold to 10-fold higher at 60°C than at 37°C Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.17
-
 ssDNA 40 mer pH 7.5, 37°C Thermus thermophilus
0.19
-
 ssDNA 50 mer pH 7.5, 37°C Thermus thermophilus
0.23
-
 ssDNA 28 mer pH 7.5, 37°C Thermus thermophilus
0.4
-
 ssDNA 21 mer pH 7.5, 37°C Thermus thermophilus
0.71
-
 ssDNA 10 mer pH 7.5, 37°C Thermus thermophilus
1
-
 ssDNA 50 mer pH 7.5, 60°C Thermus thermophilus
1.9
-
 ssDNA 28 mer pH 7.5, 60°C Thermus thermophilus
2.3
-
 ssDNA 40 mer pH 7.5, 60°C Thermus thermophilus
5.3
-
 ssDNA 21 mer pH 7.5, 60°C Thermus thermophilus
7.5
-
 ssDNA 10 mer pH 7.5, 60°C Thermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.72
-
 ssDNA 40 mer pH 7.5, 37°C Thermus thermophilus
0.83
-
 ssDNA 28 mer pH 7.5, 37°C Thermus thermophilus
1.1
-
 ssDNA 21 mer pH 7.5, 37°C Thermus thermophilus
1.7
-
 ssDNA 50 mer pH 7.5, 37°C Thermus thermophilus
2.6
-
 ssDNA 10 mer pH 7.5, 37°C Thermus thermophilus
6.4
-
 ssDNA 50 mer pH 7.5, 60°C Thermus thermophilus
7
-
 ssDNA 28 mer pH 7.5, 60°C Thermus thermophilus
7.5
-
 ssDNA 40 mer pH 7.5, 60°C Thermus thermophilus
12
-
 ssDNA 21 mer pH 7.5, 60°C Thermus thermophilus
16
-
 ssDNA 10 mer pH 7.5, 60°C Thermus thermophilus