Cloned (Comment) | Organism |
---|---|
into plasmid pcDNA3.1A(-), expressing a COOH-terminal His-Myc-tagged EXO1 protein | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F447A | abolishes binding to human MLH1 | Saccharomyces cerevisiae |
F448A | abolishes binding to human MLH1 | Saccharomyces cerevisiae |
F506A | substitution on the Mlh1 interacting protein box significantly weakens the interaction with MLH1 and has no impact on the interaction with MSH2 proteins | Homo sapiens |
F506A/F507A | substitution on the Mlh1 interacting protein box significantly weakens the interaction with MLH1 and has no impact on the interaction with MSH2 proteins | Homo sapiens |
F507A | substitution on the Mlh1 interacting protein box significantly weakens the interaction with MLH1 and has no impact on the interaction with MSH2 proteins | Homo sapiens |
additional information | exo1DELTA but not mlh1-E682A presents significant increase in canavanin-resistant mutant events. Mlh1-R547A exo1DELTA and mlh1-E682A exo1DELTA double mutants show that mlh1-R547A synergistically interacts with exo1DELTA, yielding high canavanin-resistant forward and Hom+ reversion mutation rates, whereas mlh1-E682A does not | Saccharomyces cerevisiae |
S445A | abolishes binding to human MLH1 | Saccharomyces cerevisiae |
S504A | substitution on the Mlh1 interacting protein box significantly weakens the interaction with MLH1 and has no impact on the interaction with MSH2 proteins | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | yeast Exo1 interacts with human MLH1 through its Mlh1 interacting protein box (R-SK-[Y/F]-F-motif). A mutant of MLH1 targeted at site S2 (Mlh1-E682A) behaves as a hypomorphic form of Exo1. The site S2 in Mlh1 mediates Exo1 recruitment in order to optimize mismatch repair-dependent mutation avoidance | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | yeast Exo1 interacts with human MLH1 through its Mlh1 interacting protein box (R-SK-[Y/F]-F-motif). A mutant of MLH1 targeted at site S2 (Mlh1-E682A) behaves as a hypomorphic form of Exo1. The site S2 in Mlh1 mediates Exo1 recruitment in order to optimize mismatch repair-dependent mutation avoidance | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Exo1 | - |
Homo sapiens |
Exo1 | - |
Saccharomyces cerevisiae |
exonuclease I | - |
Homo sapiens |
exonuclease I | - |
Saccharomyces cerevisiae |