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Literature summary for 3.1.11.1 extracted from

  • Dherin, C.; Gueneau, E.; Francin, M.; Nunez, M.; Miron, S.; Liberti, S.E.; Rasmussen, L.J.; Zinn-Justin, S.; Gilquin, B.; Charbonnier, J.B.; Boiteux, S.
    Characterization of a highly conserved binding site of Mlh1 required for exonuclease I-dependent mismatch repair (2009), Mol. Cell. Biol., 29, 907-918.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.11.1

Cloned(Commentary)

Cloned (Comment) Organism
into plasmid pcDNA3.1A(-), expressing a COOH-terminal His-Myc-tagged EXO1 protein Homo sapiens

Protein Variants

Protein Variants Comment Organism
F447A abolishes binding to human MLH1 Saccharomyces cerevisiae
F448A abolishes binding to human MLH1 Saccharomyces cerevisiae
F506A substitution on the Mlh1 interacting protein box significantly weakens the interaction with MLH1 and has no impact on the interaction with MSH2 proteins Homo sapiens
F506A/F507A substitution on the Mlh1 interacting protein box significantly weakens the interaction with MLH1 and has no impact on the interaction with MSH2 proteins Homo sapiens
F507A substitution on the Mlh1 interacting protein box significantly weakens the interaction with MLH1 and has no impact on the interaction with MSH2 proteins Homo sapiens
additional information exo1DELTA but not mlh1-E682A presents significant increase in canavanin-resistant mutant events. Mlh1-R547A exo1DELTA and mlh1-E682A exo1DELTA double mutants show that mlh1-R547A synergistically interacts with exo1DELTA, yielding high canavanin-resistant forward and Hom+ reversion mutation rates, whereas mlh1-E682A does not Saccharomyces cerevisiae
S445A abolishes binding to human MLH1 Saccharomyces cerevisiae
S504A substitution on the Mlh1 interacting protein box significantly weakens the interaction with MLH1 and has no impact on the interaction with MSH2 proteins Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae yeast Exo1 interacts with human MLH1 through its Mlh1 interacting protein box (R-SK-[Y/F]-F-motif). A mutant of MLH1 targeted at site S2 (Mlh1-E682A) behaves as a hypomorphic form of Exo1. The site S2 in Mlh1 mediates Exo1 recruitment in order to optimize mismatch repair-dependent mutation avoidance ?
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
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Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information yeast Exo1 interacts with human MLH1 through its Mlh1 interacting protein box (R-SK-[Y/F]-F-motif). A mutant of MLH1 targeted at site S2 (Mlh1-E682A) behaves as a hypomorphic form of Exo1. The site S2 in Mlh1 mediates Exo1 recruitment in order to optimize mismatch repair-dependent mutation avoidance Saccharomyces cerevisiae ?
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 ?

Synonyms

Synonyms Comment Organism
Exo1
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 Homo sapiens
Exo1
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 Saccharomyces cerevisiae
exonuclease I
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 Homo sapiens
exonuclease I
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 Saccharomyces cerevisiae