Literature summary for 3.1.1.B12 extracted from

Chen, S.; Pan, L.; Liu, S.; Pan, L.; Li, X.; Wang, B.
Recombinant expression and surface display of a zearalenone lactonohydrolase from Trichoderma aggressivum in Escherichia coli (2021), Protein Expr. Purif., 187, 105933 .

Search for more literature for 3.1.1.B12

Application

Application	Comment	Organism
degradation	recombinant ZHD-P can be feasible for ZEN detoxification. The recombinant Escherichia coli cells expressing ZHD-P can be applied as a whole-cell biocatalyst for ZEN detoxification	Trichoderma aggressivum

Cloned(Commentary)

Cloned (Comment)	Organism
gene zhd-p, construction of a cell surface display system for ZHD-P in Escherichia coli using pgsA as the anchoring protein gene, recombinant overexpression of the His-tagged intracellular and extracellular enzyme by Escherichia coli strain BL21(DE3)	Trichoderma aggressivum

Protein Variants

Protein Variants	Comment	Organism
additional information	the metal ions tolerance of the surface-displayed ZHD-P is better compared to the intracellular form. The surface-displayed recombinant ZHD-P can be reused four times with the residual enzyme activity of more than 50%. The biotoxicity assessment using Photobacterium phosphoreum T3 indicates that ZEN can be degraded into hypotoxic products by the intracellular or surface-displayed recombinant ZHD-P. ZHD-P can be feasible for ZEN detoxification	Trichoderma aggressivum

Inhibitors

Inhibitors	Comment	Organism	Structure
Ba2+	slight inhibition of intracellular and extracellular enzymes at 5 mM	Trichoderma aggressivum
Ca2+	significant inhibition at 5 mM of intracellular enzyme	Trichoderma aggressivum
Co2+	significant inhibition of the intracellular enzyme, and slight inhibition of extracellular enzyme at 5 mM	Trichoderma aggressivum
Cu2+	significant inhibition of intracellular and extracellular enzymes at 5 mM	Trichoderma aggressivum
K+	slight inhibition at 5 mM of intracellular enzyme	Trichoderma aggressivum
Mg2+	slight inhibition at 5 mM of intracellular enzyme	Trichoderma aggressivum
Mn2+	significant inhibition at 5 mM of intracellular enzyme	Trichoderma aggressivum
Na+	slight inhibition at 5 mM of intracellular enzyme	Trichoderma aggressivum
Ni2+	significant inhibition of intracellular and extracellular enzymes at 5 mM	Trichoderma aggressivum
Zn2+	significant inhibition of intracellular and extracellular enzymes at 5 mM	Trichoderma aggressivum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	slight activation of extracellular enzyme at 5 mM	Trichoderma aggressivum
K+	slight activation of extracellular enzyme at 5 mM	Trichoderma aggressivum
Mg2+	slight activation of extracellular enzyme at 5 mM	Trichoderma aggressivum
Mn2+	slight activation of extracellular enzyme at 5 mM	Trichoderma aggressivum
additional information	the metal ions tolerance of the surface-displayed ZHD-P is better compared to the intracellular form	Trichoderma aggressivum
Na+	slight activation of extracellular enzyme at 5 mM	Trichoderma aggressivum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
zearalenone + H2O	Trichoderma aggressivum	-	2,4-dihydroxy-6-[(1E,10S)-10-hydroxy-6-oxoundec-1-en-1-yl]-benzoic acid	-	?

Organism

Organism	UniProt	Comment	Textmining
Trichoderma aggressivum	W0M1M6	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged intracellular (1.55fold) and extracellular enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Trichoderma aggressivum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
124.19	-	crude recombinant, intracellular enzyme, pH 9.0, 45°C, substrate ZEN	Trichoderma aggressivum
191.94	-	purified recombinant, intracellular enzyme, pH 9.0, 45°C, substrate ZEN	Trichoderma aggressivum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
zearalenone + H2O	-	Trichoderma aggressivum	2,4-dihydroxy-6-[(1E,10S)-10-hydroxy-6-oxoundec-1-en-1-yl]-benzoic acid	-	?

Synonyms

Synonyms	Comment	Organism
ZHD	-	Trichoderma aggressivum
ZHD-P	-	Trichoderma aggressivum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	recombinant extracellular enzyme	Trichoderma aggressivum
45	-	recombinant intracellular enzyme	Trichoderma aggressivum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
25	40	the recombinant intracellular ZHD-P retains 100% activity when it is incubated at 25-40°C for 1 h	Trichoderma aggressivum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	9	recombinant intracellular enzyme	Trichoderma aggressivum
9	-	recombinant extracellular enzyme, displays maximal activity at pH 9.0, but the activity decreases sharply when pH is higher than 9.0	Trichoderma aggressivum

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	10	intracellular ZHD-P retains 60% of the maximum enzyme activity within this range. ZHD-P is a neutral ZEN-degrading enzyme	Trichoderma aggressivum

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	11	the surface-displayed recombinant ZHD-P retains 80% activity when it is incubated at pH 5.0-11.0 for 12 h. 80% of the intracellular enzyme activity is retained after incubating for 12 h at pH 7.0-9.0	Trichoderma aggressivum

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Release 2023.1 (January 2023)